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Modified Organism
Potato with altered growth and tuber quality
Record information and status
Record ID
100277
Status
Published
Date of creation
2009-07-07 15:00 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-20 14:58 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-20 14:58 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with altered growth and tuber quality
Transformation event
Potato transformed with B33-Apy1-RNAi 1331 (3 lines: #3, #10 und #25)
Developer(s)
Max-Planck-Institute of Molecular Plant Physiology
Wissenschaftspark Golm
Am Mühlenberg 1
14476 Potsdam - Golm
Germany
Description
Potato plant was modified with an inverted repeat RNAi construct of the Solanum tuberosum Apyrase gene along with an nptII expression cassette. The RNAi construct results in the degradation of native apyrase RNA transcript thus reducing the expression of the enzyme. This results in the modfied growth of potato tubers expressing the gene. The nptII gene conferred resistance to kanamycin and was used as a selection marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
B33-Apy1-RNAi 1331
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
B33 gene promotor
0.00 Kb
 
 
Apyrase-gene
0.00 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.00 Kb
 
 
Apyrase-gene
0.00 Kb
 
 
Octopine Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Using the RNAi construct, the RNA of a part of the apyrase gene is coded in sense and antisense orientation, so that the sense and antisense RNA are separated by a sufficiently long spacing sequence (RNA of the Pdk intron from Flaveria trinervia).

In the present case RNAi synthesis takes place under the control of the B33 promoter specifically in the potato tubers. The sense and antisense RNAi fractions form a double strand, the individual strands of which are linked by a hairpin loop.

This double-stranded RNA is recognised by specific enzymes in the plant cell and is fractionated into small fragments. These bind to the mRNA of the respective gene (the apyrase gene) and mediate the decomposition of the RNA by the same enzymes.
LMO characteristics
Modified traits
  • Other growth, development and product quality
How the expression of the gene(s) was affected
Silencing of the endogenous apyrase gene applying RNAi technology.
Common use(s)
  • Food
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record