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Modified Organism
Pea modified for increased protein content
Record information and status
Record ID
100288
Status
Published
Date of creation
2009-07-20 13:33 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-19 19:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-19 19:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Pea modified for increased protein content
Transformation event
Lines 14/10 and 14/3
Developer(s)
Institute for Plant Genetics and Crop Plant Research (IPK-Gatersleben)
Corrensstrasse 3
Gatersleben
D-06466
Germany
Url:www.ipk-gatersleben.de
Description
The Vfaap1 gene from Vicia faba encodes an amino acid permease. Amino acid permeases transport amino acids from the plant vascular tissue into the symplasts of plant cells. They are differentially expressed according to plant development and tissue, and exhibit a range of specificity patterns for amino acids. VfAAP1 mediates the transport of a range of amino acids (mainly cysteine, arginine, glutamine, serine, leucine, methionine, histidine, glycine and threonine) with particular emphasis on cysteine. The formation of the amino acid permease of the field bean in pea seeds and the results in an increase in the seed protein content.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Pisum sativum - Garden pea, PEA
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: Eiffel
Characteristics of the transformation process
Vector
pPZP200/VfAAP1 and pCAMBIA3300D
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Legumin B4 gene promoter
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Amino acid permease 1 gene
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Octopine Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Notes about the Transcriptional regulator(s) specific to this LMO
The legumin B4 gene (leB4 gene) promoter from Vicia faba regulates the seed-specific expression of genes under its control.

Notes about the Other(s) sequence(s) specific to this LMO
The genetically modified peas were developed applying a co-transformation approach with the gene of interest (Vfaap1-gene) and the marker gene for selection of the transformed plant tissue (bar-gene) being located on two different plasmids pPZP200/VfAAP1 and pCAMBIA3300D respectively. As a result of the integration of the T-DNAs of both transformation plasmids at different locations in the plant genome and as a result of the breeding steps taken following regeneration of the fertile plants, the bar gene is no longer contained in the plants intended for release.
LMO characteristics
Modified traits
Common use(s)
  • Feed
  • Research
Detection method(s)
Additional information
Expression of the Vfaap1 gene is controlled by the promoter of the legumin B4 gene (leB4 gene) from Vicia faba. This promoter regulates the seed-specific expression of the genes under its control.  Expression in pollen has also been observed. In immature seeds of the transgenic pea line proposed for release, expression of the gene was verified by Northern blot. Other parts of the plant have not yet been examined for expression.
Additional Information
Additional Information
In greenhouse studies on the genetically modified pea line 14/10 no differences in habitus (growth and flowering time) were found in comparison to the control line 14/3. The number and weight of the seeds and their protein content as well as the C:N ratio were also defined. With the exception of an increase in protein content, the genetically modified plants displayed normal phenotype.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record