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Modified Organism
Potato modified for increased Stomata Density
Record information and status
Record ID
100293
Status
Published
Date of creation
2009-07-20 15:57 UTC (german_bch@bvl.bund.de)
Date of last update
2013-03-11 14:26 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-11 14:26 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for increased Stomata Density
Transformation event
Lines: 2, 6, 12
Developer(s)
Max Planck Institute of Molecular Plant Physiology (MPIMP)
Max-Planck-Institut für Molekulare Pflanzenphysiologie
Am Mühlenberg 1
Potsdam
Germany, 14476
Phone:+49 331 567 - 80
Fax:+49 331 567 - 84 08
Email:contact@mpimp-golm.mpg.de
Url:Max-Planck-Institut für Molekulare Pflanzenphysiologie
Description
Transgenic potatoes expressing an RNAi construct against the endogenous StSDD1 gene. The resulting plant exhibits an increase in stomata density by up to 25%. Thereby these plants have enhanced transpiration activity, decreased leaf roll at high light intensities, and increased tuber yield.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Désirée
Related LMOs
Potato modified for decreased Stomata Density
Resistance to antibiotics - Hygromycin Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
pBinAR-StSDD1 (Derived from pBIN19)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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Stomatal density and distribution 1 gene
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Stomatal density and distribution 1 gene
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Octopine Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transgenic potatoes pBINAR-StSDD1 contain the StSDD1 RNAi construct consisting of the CaMV 35S promoter, a fragment of the endogenous StSDD1 gene in sense and antisense orientation separated by a part of the multiple cloning site of the vector pCR2.1-TOPO and the ocs terminator that mediates increased formation of stomata in leaves. These potatoes further encode an nptII cassette as a selection marker system that confers tolerance to aminoglycoside antibiotics (kanamycin, neomycin, geneticin). Here, the nptII gene is put under the control of the nos promoter and -terminator.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Stomatal density and distribution 1 gene - Solanum tuberosum - Potato, SOLTU
Tolerance to abiotic stress - Drought
How the expression of the gene(s) was affected
In order to increase guard cell density, the expression of the potato's own SDD1 protein was reduced in these potato plants with the aid of an RNAi construct (pBinAR-StSDD1). Using the RNAi construct, the RNA of a part of the SDD1 gene is transcribed in sense and antisense orientation so that sense and antisense RNA are separated by a sufficiently long spacing sequence. The sense and antisense sections of the RNAi form a RNA doublestrand. The RNA double strand is recognized by specific enzymes in the plant cell and broken down into small fragments. These RNA fragments adhere to the mRNA of the endogenous SDD1 gene and activate the degradation of the mRNA.
Common use(s)
  • Research

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record