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Modified Organism
Potato modified for decreased Stomata Density
Record information and status
Record ID
100294
Status
Published
Date of creation
2009-07-20 16:29 UTC (german_bch@bvl.bund.de)
Date of last update
2013-03-11 14:21 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-11 14:21 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for decreased Stomata Density
Transformation event
Lines: 1, 2, 7, 9, 11
Developer(s)
Max-Planck-Institute of Molecular Plant Physiology
Wissenschaftspark Golm
Am Mühlenberg 1
14476 Potsdam - Golm
Germany
Description
This transgenic potato exhibits a reduced amount of stomata (reduced by up to 55% compared to wild-type) by over expressing the SDD1 protein which decreases guard cell density This results in increased leaf roll at high light intensities and decreased tuber yield.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Related LMOs
Potato modified for increased Stomata Density
Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
pBinARHyg-AtSDD1
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Stomatal density and distribution 1 gene
0.00 Kb
 
 
Octopine Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
In order to decrease guard cell density, a construct (pBinARHyg-AtSDD1) was transferred that causes the potato plants to overexpress the SDD1 protein from Arabidopsis thaliana. AtSDD1 is put under the control of the CaMV 35S promoter and the ocs terminator. An hph gene cassette consisting of the nos promoter, the hph gene, the polyadenylation signal of gene 7 from plasmid pTiAch5 from Agrobacterium tumefaciens, and the nos terminator was used as a selection marker system.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record