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Modified Organism
BCS-GHØØ4-7 - Herbicide-tolerant, insect-resistant cotton
Record information and status
Record ID
101018
Status
Published
Date of creation
2010-03-04 05:16 UTC (bch@env.go.jp)
Date of last update
2014-02-12 17:15 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-12 17:15 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant, insect-resistant cotton
Transformation event
T304-40
Unique identifier
BCS-GHØØ4-7
Developer(s)
Bayer Crop Science K.K
Marunouchi Kitaguchi Building, 1-6-5, Marunouchi
Chiyoda-ku, Tokyo
Japan
Url:http://www.bayercropscience.co.jp/
Description
LM cotton line, T304-40, is protected against feeding damage by Lepidopteran insect larvae, and is also tolerant to herbicides containing glufosinate ammonium. Insect protection is conferred by expression of a modified Cry1Ab protein from Bacillus thuringiensis and herbicide tolerance is conferred by expression of phosphinothricin acetyltransferase (PAT) from Streptomyces hygroscopicus.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Characteristics of the transformation process
Vector
pTDL008 derived from pGSV20
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
NADP-malic enzyme 1 gene 3’UTR and terminator
0.94 Kb
 
 
Cry1Ab
1.85 Kb
 
 
5'e1 Leader
0.06 Kb
 
 
Ps7s7
1.04 Kb
 
 
CaMV 35S promoter
0.86 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.31 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified cry1Ab gene1 has been derived from a gene (Genbank accession No. X04698 - first cloned and characterised by Höfte et al (1986)) which, under the latest nomenclature system, is now known as cry1Ab5 (Bacillus thuringiensis toxin nomenclature,database available online at http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/toxins2.html).

Sequencing of the 9056 bp inserted transgenic construct and Southern blot analysis revealed an almost full copy of the T-DNA construct (with an incomplete 3`me1 terminator) was inserted into the T304-40 LM cotton line in addition to:

• a partial 3'me1 terminator;
• a partial copy of the cry1Ab gene cassette, with a trunckated Ps7s7 promoter, in a tail-to-tail orientation, and
• a partial copy of the bar gene cassette in which the nos terminator is truncated.

As a result of the transformation event, four new junctions have been created (see Figure 4 in the attached file below), two being located at the 5' and 3' ends of the insert and two being located within the insert as a result of the rearrangement. Southern blot analysis revealed no vector backbone sequences in cotton line T304-40.

For further description of the T304-40 LM cotton, see the safety assessment document attached below.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
  • Industrial

Records referencing this document (27)
IDDescription
27record(s) found
Country's Decision or any other Communication10 records
Modified Organism6 records
Organization1 record
Risk Assessment10 records