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Modified Organism
Poplar modified for increased glutathione content
Record information and status
Record ID
101272
Status
Published
Date of creation
2010-07-19 15:55 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-10 19:31 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-10 19:31 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Poplar modified for increased glutathione content
Transformation event
ggs11
Developer(s)
Albert-Ludwigs-Universität Freiburg
Institut für Forstbotanik und Baumphysiologie [Institute of Forest Botany and Tree Physiology]
Albert-Ludwigs-Universität Freiburg (ALU)
Georges-Köhler-Allee 53/54
Freiburg, Baden-Württemberg
Germany, 79110
Phone:+49 761 203-8300
Fax:+49 761 203-8302
Email:sabine.pregger@ctp.uni-freiburg.de,annette.schlierenkamp@ctp.uni-freiburg.de
Url:University of Freiburg, Chair of Tree Physiology
Description
Populus x canescens was transformed with the E.coli γ-glutamylcysteine synthetase gene resulting in the increased synthesis of glutathione in the cytosol of the plant's leaves. This was done to examine the oxidative stress tolerance capacity and possible detoxification capacity against various environmental pollutants exhibited by Populus x canescens expressing this modified genotype.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus alba - Gray Poplar
Characteristics of the transformation process
Vector
p70gshl, developed from pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid left border repeat
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Gamma-glutamylcysteine Synthetase I gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Ti plasmid right border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
To transform the grey poplar the start codon of the endogenous gshI gene from E. coli was modified from TTG to ATG in the p70gshl construct. This causes an amino acid exchange from leucine to methionine.

Notes about the Other(s) sequence(s) specific to this LMO

The HindIII/SmaI fragment containing the gshI coding sequence (1.7 kb) was inserted into the same sites of the plasmid pLBR19, which contains the enhanced CaMV 35S promoter (P-e35S).

The P-e35S - gshI - T-35S cassette was cloned as an SstI/XbaI fragment into the binary vector pBIN 19 to create p70gshI.

Poplar line ggs11 possesses two copies of the DNA cassette.

LMO characteristics
Modified traits
Common use(s)
  • Timber
Additional Information
Additional Information
For a detailed description of the modification of poplar line ggs11 see article by Arisi et al. (1997) below.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record