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Modified Organism
Dominant lethal Aedes aegypti mosquito
Record information and status
Record ID
101474
Status
Published
Date of creation
2010-12-14 11:45 UTC (anita@nre.gov.my)
Date of last update
2013-09-17 20:01 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-17 20:01 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Dominant lethal Aedes aegypti mosquito
Transformation event
OX513A(My1) (AKA LA513A)
Developer(s)
Oxitec Limited
71 Milton Park
OX14 4RX
Oxford, England
Phone:+44 (0) 1235 832393
Fax:+44 (0) 1235 861138
Email:info@oxitec.com
Url:http://www.oxitec.com
Dr. Lee Han Lim
Medical Entomologist
Unit of Medical Entomology
Institute for Medical Research (IMR)
Jalan Pahang
Kuala Lumpur
Malaysia, 50588
Phone:+603-2616-2666
Fax:+603-2693-9335
Email:leehl@imr.gov.my
Url:http://www.imr.gov.my
Description
A transgenic strain of the Aedes aegypti mosquito, designated as OX513A(My1), was developed to exhibit dominant lethality in both males and females when reared in the absence of a transgene repressor as well as a dominant fluorescent marking to identify the transgenic insects.

Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Aedes aegypti - Yellow fever mosquito, AEDAE
Point of collection or acquisition of the recipient organism
Aedes aegypti of the Rockefeller strain
Characteristics of the transformation process
Vector
pLA513 and phsp-pBac
Techniques used for the modification
  • Microinjection
Genetic elements construct
 
Actin 5c gene Promotor
0.00 Kb
 
 
DsRed2 Fluorescent Protein
0.00 Kb
 
 
Dorsomycin gene 3'UTR
0.00 Kb
 
 
tet Operator Sequences
0.00 Kb
 
 
HSP70 minimal promoter
0.00 Kb
 
 
Tetracycline-controlled transactivator
0.00 Kb
 
 
fs(1)K10 3' UTR
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The pLA513 plasmid was co-transformed with the phsp-pBac helper plasmid which served as a source for the piggy Bac transposase.
LMO characteristics
Modified traits
Common use(s)
  • Biological control
Additional Information
Additional Information
Information on OX513A(My1)

OX513A(My1) is a bisex RIDL strain, which means that both female and male insects die unless supplied with the supplement, which in the case of OX513A(My1) is the antibiotic tetracycline.

Released bisex RIDL insects and their progeny die within a few weeks so releases must be sustained to maintain the control.

Source: Oxitec (see developer field above).

Information on the Release of Insects carrying a Dominant Lethal (RIDL) technology

Release of Insects carrying a Dominant Lethal (RIDL) is a method using recombinant DNA technology to create genetically modified insects for biological control. The dominant lethal gene kills the insects but it can be repressed by an external additive, which allows the insects to be reared in manufacturing facilities. This external additive is commonly administered orally, and so can be an additive to the insect food. The insects can also be given genetic markers, such as fluorescence, that make monitoring the progress of eradication easier.

There are potentially several types of RIDL, but the more advanced forms have a female-specific dominant lethal gene. This avoids the need for a separate sex separation step, as the repressor can be withdrawn from the final stage of rearing, leaving only males.

These males are then released in large numbers into the affected region. The released males are not sterile, but any female offspring their mates produce will have the dominant lethal gene expressed, and so will die. The number of females in the wild population will therefore decline, causing the overall population to decline.

Using RIDL means that the males will not have to be sterilized by radiation before release (as done with the "Sterile Insect Technique" (SIT) using radiation), making the males healthier when they need to compete with the wild males for mates.

Source: Wikipedia (see link below).

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication2 records
Information Resource1 record
Modified Organism1 record
Risk Assessment2 records