Brassica napus modified for the synthesis of resveratrol | BCH-LMO-SCBD-101525 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-101525-3   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
published: 20 Jan 2011 last updated: 06 Sep 2012
Living Modified Organism identity
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Brassica napus modified for the synthesis of resveratrol
EN
pPSty5 (line 1104.3.6)
No
Brassica napus is modified with the VST1 gene which codes for stilbene synthase. This enzyme catalyses the a step in the reaction that leads to the synthesis of resveratol in Brassica napus.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line: Drakkar, Lisora
EN
Characteristics of the modification process
pSty5 derived from pPZP111
EN
  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101520-4 Stilbene Synthase | Vitis vinifera (Grape Vine)
    Protein coding sequence | Changes in quality and/or metabolite content (Antioxidants, Flavonoids (e.g. anthocyanin)),Resistance to diseases and pests (Fungi),Tolerance to abiotic stress
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-101521-6 Napin gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Promoter
  • BCH-GENE-SCBD-103887-1 Stilbene Synthase Terminator | Vitis vinifera (Grape Vine)
    Terminator
The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
EN
LMO characteristics
EN
  • Research
Detection method(s)
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Additional Information
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