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Modified Organism
Brassica napus modified for the synthesis of resveratrol
Record information and status
Record ID
101525
Status
Published
Date of creation
2011-01-20 12:54 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-06 18:16 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 18:16 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Brassica napus modified for the synthesis of resveratrol
Transformation event
pPSty5 (line 1104.3.6)
Developer(s)
FINAB e. V.
Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)
Thünenplatz 1
Groß Lüsewitz, Mecklenburg-Vorpommern
Germany, 18190
Phone:+49 (0)381 498 30-81
Email:webmaster@finab.de
Url:FINAB e. V.
Description
Brassica napus is modified with the VST1 gene which codes for stilbene synthase. This enzyme catalyses the a step in the reaction that leads to the synthesis of resveratol in Brassica napus.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Drakkar, Lisora
Characteristics of the transformation process
Vector
pSty5 derived from pPZP111
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Napin gene promoter
0.00 Kb
 
 
Stilbene Synthase
0.00 Kb
 
 
Stilbene Synthase Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record