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Modified Organism
Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)
Record information and status
Record ID
101526
Status
Published
Date of creation
2011-01-20 13:00 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-06 19:03 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 19:03 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)
Transformation event
pLH-BnSGT-GUS (line 1501.24)
Developer(s)
FINAB e. V.
Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)
Thünenplatz 1
Groß Lüsewitz, Mecklenburg-Vorpommern
Germany, 18190
Phone:+49 (0)381 498 30-81
Email:webmaster@finab.de
Url:FINAB e. V.
Description
In the GM oilseed rape plants that were transformed using the construct pLH-BnSGT-GUS the activity of the native oilseed rape enzyme UDP-glucose:sinapate glucosyltransferase (SGT) was reduced with the help of an RNAi construct. As a step in the biosynthesis of sinapine the UDP-glucose:sinapate glucosyltransferase catalyses the conversion of sinapic acid to sinapoyl glucose.

Suppression of the UDP-glucose:sinapate glucosyltransferase leads to a reduction of sinapine content in the seeds of the GM oilseed rape plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Drakkar, Lisora
Characteristics of the transformation process
Vector
pLH-BnSGT-GUS Agrobacterium Ti plasmid derived
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
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CaMV 35S terminator
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Napin gene promoter
0.00 Kb
 
 
UDP-glucose:sinapate glucosyltransferase
0.00 Kb
 
 
UDP-glucose:sinapate glucosyltransferase
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record