Record details

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Living Modified Organism identity

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LMO name

Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)

Transformation event

pLH-BnSGT-GUS (line 1501.24)

Developer(s)

FINAB e. V.

Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)

Thünenplatz 1

Groß Lüsewitz, Mecklenburg-Vorpommern

Germany, 18190

Phone:	+49 (0)381 498 30-81
Email:	webmaster@finab.de
Url:	FINAB e. V.

Record #101524

Description

In the GM oilseed rape plants that were transformed using the construct pLH-BnSGT-GUS the activity of the native oilseed rape enzyme UDP-glucose:sinapate glucosyltransferase (SGT) was reduced with the help of an RNAi construct. As a step in the biosynthesis of sinapine the UDP-glucose:sinapate glucosyltransferase catalyses the conversion of sinapic acid to sinapoyl glucose.

Suppression of the UDP-glucose:sinapate glucosyltransferase leads to a reduction of sinapine content in the seeds of the GM oilseed rape plants.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape

Record #12083

Point of collection or acquisition of the recipient organism

Cultivar/breeding line: Drakkar, Lisora

Characteristics of the transformation process

Vector

pLH-BnSGT-GUS Agrobacterium Ti plasmid derived

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV 35S promoter

#100287

0.00 Kb

Bialaphos resistance gene

#14972

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

Napin gene promoter

#101521

0.00 Kb

UDP-glucose:sinapate glucosyltransferase

#101523

0.00 Kb

UDP-glucose:sinapate glucosyltransferase

#101523

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)

LMO characteristics

Modified traits

Changes in quality and/or metabolite content
- Protein and amino acids
Resistance to herbicides
- Glufosinate
Selectable marker genes and reporter genes

Common use(s)

Research