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Modified Organism
Brassica napus modified for reduced sinapine content (pLH7000-SGT/SCT)
Record information and status
Record ID
101527
Status
Published
Date of creation
2011-01-20 13:18 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-06 19:05 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 19:05 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Brassica napus modified for reduced sinapine content (pLH7000-SGT/SCT)
Transformation event
pLH7000-SGT/SCT (line 1505.1d)
Developer(s)
FINAB e. V.
Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)
Thünenplatz 1
Groß Lüsewitz, Mecklenburg-Vorpommern
Germany, 18190
Phone:+49 (0)381 498 30-81
Email:webmaster@finab.de
Url:FINAB e. V.
Description
In the GM oilseed rape plants that were transformed using the construct pLH7000-SGT/SCT, the activity of the native oilseed rape enzymes UDP-glucose:sinapate glucosyltransferase (SGT) and sinapoylglucose:choline sinapoyltransferase (SCT) was reduced with the help of an RNAi construct. In oilseed rape plants UDP-glucose:sinapate glucosyltransferase catalyses the conversion of sinapic acid to sinapoylglucose. Sinapoylglucose:choline sinapoyl-transferase (SCT) catalyses the conversion of sinapoylglucose to sinapoyl-choline (sinapine).

By suppressing UDP-glucose:sinapate glucosyltransferase and sinapoylglucose:choline si-napoyltransferase the sinapine content in the seeds of the GM oilseed rape plants is further reduced.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Drakkar, Lisora
Characteristics of the transformation process
Vector
pLH7000-SGT/SCT
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Napin gene promoter
0.00 Kb
 
 
Sinapoylglucose:choline sinapoyltransferase
0.20 Kb
 
 
UDP-glucose:sinapate glucosyltransferase
0.18 Kb
 
 
UDP-glucose:sinapate glucosyltransferase
0.18 Kb
 
 
Sinapoylglucose:choline sinapoyltransferase
0.20 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNAi construct:
- seed specific short napin 590-promoter
- 197 bp fragment of the cDNA of the SCT-gen and 183 bp of the cDNA of the SGT-gen in sense orientation,
- nt790-nt1812 of uidA as a spacer, and 183 bp of the cDNA of the SGT-gen and 197 bp of the cDNA of SCT-gen in antisense orientation
- terminator of the nos gene.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record