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Modified Organism
Brassica napus modified for the synthesis of resveratrol and reduced sinapine content
Record information and status
Record ID
101528
Status
Published
Date of creation
2011-01-20 13:52 UTC (german_bch@bvl.bund.de)
Date of last update
2012-09-06 19:57 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 19:57 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Brassica napus modified for the synthesis of resveratrol and reduced sinapine content
Transformation event
pPSty5 / pLH-BnSGT-GUS (line 1502.15.7)
Developer(s)
FINAB e. V.
Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)
Thünenplatz 1
Groß Lüsewitz, Mecklenburg-Vorpommern
Germany, 18190
Phone:+49 (0)381 498 30-81
Email:webmaster@finab.de
Url:FINAB e. V.
Description
The aim is to reduce the sinapine content and at the same time to synthesise resveratrol in the GM plants by combining the suppression cassette for the UDP-glucose:sinapate glucosyltransferase (SGT) gene with the gene that encodes the stilbene synthase VST I. In order to achieve this, the oilseed rape plants were co-transformed using the constructs pPSty5 and pLH-BnSGT-GUS.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Drakkar, Lisora
Related LMOs
Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)
FINAB e. V. Changes in quality and/or metabolite content - Protein and amino acids Resistance to herbicides - Glufosinate
Brassica napus modified for the synthesis of resveratrol
FINAB e. V. Changes in quality and/or metabolite content - Antioxidants, Flavonoids (e.g. anthocyanin) Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Fungi Tolerance to abiotic stress
Characteristics of the transformation process
Vector
pPSty5 and pLH-BnSGT-GUS
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Napin gene promoter
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Stilbene Synthase
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Stilbene Synthase Terminator
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CaMV 35S promoter
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Neomycin Phosphotransferase II
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CaMV 35S terminator
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CaMV 35S promoter
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Phosphinothricin N-acetyltransferase gene
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CaMV 35S terminator
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Napin gene promoter
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UDP-glucose:sinapate glucosyltransferase
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UDP-glucose:sinapate glucosyltransferase
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Information Regarding pPSty5
The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.

Information Regarding pLH-BnSGT-GUS
The plasmid used to transform the oilseed rape plants with the construct pLH-BnSGT-GUS and is derived from the binary vector pLH7000 (Hausmann and Töpfer, 1999) and contains the following genetic elements outside the border regions:
- the aadA gene for resistance to the antibiotics streptomycin and spectinomycin;
- the bom sequence and the nic sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.
As a rule, in Agrobacterium-mediated transformations only DNA fragments located between the border regions are integrated into the plant genome. However, in isolated cases the transfer of DNA fragments located outside the border regions has been reported and cannot be ruled out entirely.
-RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record