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Modified Organism
SYN-BTØ11-1 x SYN-IR162-4 x DAS-Ø15Ø7-1 x MON-ØØØ21-9 - Agrisure™ Viptera 3220 Maize
Record information and status
Record ID
101785
Status
Published
Date of creation
2011-04-18 06:49 UTC (bch@env.go.jp)
Date of last update
2013-07-24 17:14 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 17:14 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Agrisure™ Viptera 3220 Maize
Transformation event
Bt11 x MIR162 x 1507 x GA21
Unique identifier
SYN-BTØ11-1 x SYN-IR162-4 x DAS-Ø15Ø7-1 x MON-ØØØ21-9
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Description
The stacked maize line SYN-BTØ11-1 x SYN-IR162-4 x DAS-Ø15Ø7-1 x MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of Cry1Ab, Cry1F, PAT, Vip3Aa, EPSPS and PMI genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide and allows the LMO to use mannose as a carbon source.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
SYN-IR162-4 - Agrisure™ Viptera maize
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Related LMOs
SYN-BTØ11-1 x MON-ØØØ21-9 - YieldGard™ Roundup Ready™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate, Glyphosate
Show detection method(s)
SYN-BTØ11-1 x SYN-IR162-4 x MON-ØØØ21-9 - Agrisure® Viptera™ 3110 Maize
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate, Glyphosate Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pZO1502, pNOV1300, PHI8999A and pDPG434
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV 35S promoter
0.51 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.47 Kb
 
 
Cry1Ab
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Alcohol Dehydrogenase 1, intron 2
0.18 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Vegetative insecticidal protein 3Aa20
2.37 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from GA21, vector pDPG434
The GA21 line of maize was genetically engineered, by particle acceleration (biolistic) transformation, to be tolerant of glyphosate-containing herbicides.

DNA insert from Bt 11 vector pZO1502
Maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR162 vector pNOV1300
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  i it also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed

Records referencing this document (9)
IDDescription
9record(s) found
Country's Decision or any other Communication4 records
Information Resource1 record
Risk Assessment4 records