Agrisure™ Viptera 3220 Maize

The stacked maize line SYN-BTØ11-1 x SYN-IR162-4 x DAS-Ø15Ø7-1 x MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of Cry1Ab, Cry1F, PAT, Vip3Aa, EPSPS and PMI genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide and allows the LMO to use mannose as a carbon source.

pZO1502, pNOV1300, PHI8999A and pDPG434

DNA insert from GA21, vector pDPG434
The GA21 line of maize was genetically engineered, by particle acceleration (biolistic) transformation, to be tolerant of glyphosate-containing herbicides.

DNA insert from Bt 11 vector pZO1502
Maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR162 vector pNOV1300
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  i it also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMOs.