Herbicide-tolerant, insect-resistant cotton

The stacked cotton line  was generated through the traditional cross breeding of parental lines GHB614, LLCotton25 and 15985 to express the cry1A(c), cry2A(b), 2mepsps and the bar genes. The expression of these genes confers resistance against lepidoptera pests and tolerance to glyphosate and glufosinate herbicides.

PV-GHBK11, PV-GHBK04, pTEM2 and pGSV71

DNA insert from 15985 vector PV-GHBK11
The T-DNA from this vector contributed the coding sequences of the cry2A(b) and uidA genes. These allow for the expression of the insecticidal cry2A(b) crystal protein which protects against lepidoptera pests and the Beta-Glucuronidase selectable marker.

DNA insert from 15985 vector PV-GHBK04
Originally integrated into the MON531 line and persisted through to the 15985 line. The T-DNA from this vector contains the coding sequence of the cry1A(c)  gene and the nptII resistance gene. The aadA gene was also integrated into the host genome however it is not expressed.

DNA insert from GHB614 vector pTEM2
Cotton event GHB614 contains a stably integrated 2mepsps gene. This modification confers to the protein a decreased binding affinity for the herbicide glyphosate, allowing it to maintain sufficient enzymatic activity and thus tolerance in its presence.

DNA insert from LLCotton25 vector pGSV71
Cotton tolerant to glufosinate ammonium herbicide produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (bar) from the soil bacterium Streptomyces hygroscopicus.