SBVR111 rhizomania resistant sugar beet | BCH-LMO-SCBD-101876 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
published: 09 Jun 2011 last updated: 20 Sep 2012
Living Modified Organism identity
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SBVR111 rhizomania resistant sugar beet
EN
SBVR111 (previously known as GM RZ 13)
No
SBVR111 sugar beet expresses the RZM gene which confers resistance to rhisomania disease caused by the beet necrotic yellow vein virus (BNYVV) through interaction with the reproductive system of the virus that will lead to a reduction of the development of the virus in the plant.

SBVR111 sugar beet also expresses a Phosphomannose Isomerase (PMI) protein, which acts as a selectable marker enabling transformed plant cells to utilize mannose as a primary carbon source.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pSYN15965 (formerly known as pHiNK188)
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101874-2 Ubiquitin gene 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-101870-7 RZM Genetic Element | Beet necrotic yellow vein virus (BNYVV)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Beet necrotic yellow virus (BNYV))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101873-3 Heatshock protein 80 gene promoter | Brassica oleracea (Wild cabbage, Crucifers, BRAOL)
    Promoter
  • BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
The part intended for the insertion contains RZM gene originated from the Beet Necrotic Yellow Vein Virus (BNYVV). The gene RZM is the inverted repeat of a part of the RNA-1 gene transcript of the BNYVV. This portion of RNA-1 encodes the RNA dependent RNA polymerase (RdRp) or replicase protein. Expression of the RZM, driven by the promoter and intron from the Ubiquitin 3 (Ubi3) gene of Arabidopsis thaliana, confers resistance to BNYVV by targeting the replicase RNA transcript of the infecting virus via the RNAi mechanism.
EN
LMO characteristics
EN
  • Food
Detection method(s)
Data from Southern analysis show that SBVR111 sugar beet contains only one copy of the tested transgene. SVBR111 does not contain any replication sequence from the transformation plasmid pSYN15965. Moreover, the statistic analysis of segregation ratios over several generations confirmed an expected Mendel´s ratio for pmi gene. Data show that the insert was integrated into SBVR111 sugar beet nuclear genome.
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism” or “Parental Organisms 1
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1