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Modified Organism
Rhizomania resistant, glyphosate tolerant sugar beet
Record information and status
Record ID
101878
Status
Published
Date of creation
2011-06-06 14:18 UTC (hana_jirakova@env.cz)
Date of last update
2015-11-23 20:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-11-23 20:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rhizomania resistant, glyphosate tolerant sugar beet
Transformation event
SBVR111 x H7-1
Developer(s)
Syngenta
Syngenta
Research Triangle Park, North Carolina
United States of America
Phone:919-541-8500
Email:http://www.syngentabiotech.com/contact/contactus.aspx
Url:Syngenta
Description
SBVR111 x H7-1 sugar beet is produced by conventional breeding crosses of  SBVR111 and H7-1 genetically modified sugar beet, and therefore expresses transgenic proteins inherited from single GM sugar beet parent events (from H7-1: CP4 EPSPS, from SBVR111: RZM and PMI).

SBVR111 and H7-1 sugar beet confer resistance to Rhizomania and tolerance to herbicide products containing glyphosate, respectively.

Rhizomania is a disease in sugar beet that is spreading rapidly and is induced by the Beet Necrotic Yellow Vein Virus (BNYVV). SBVR111 sugar beet expresses a gene (RZM) which confers resistance to the disease through interaction with the reproductive system of the virus that will lead to a reduction of the development of the virus in the plant. SBVR111 sugar beet also expresses a Phosphomannose Isomerase (PMI) protein, which acts as a selectable marker enabling transformed plant cells to utilize mannose as a primary carbon source.

H7-1 sugar beet expresses the CP4 EPSPS enzyme which confers the tolerance to glyphosate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
SBVR111 rhizomania resistant sugar beet
Mannose tolerance Resistance to diseases and pests - Viruses - Beet necrotic yellow virus (BNYV) Selectable marker genes and reporter genes
Show detection method(s)
KM-ØØØH71-4 - Roundup Ready™ sugar beet
Resistance to herbicides - Glyphosate
Show detection method(s)
Beta vulgaris - Common beet, Sugarbeet, BETMA
Characteristics of the transformation process
Vector
pSYN15965 and PV-BVGT08
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene 3 promoter
0.00 Kb
 
 
RZM Genetic Element
0.00 Kb
 
 
RZM Genetic Element
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Heatshock protein 80 gene promoter
0.00 Kb
 
 
Phosphomannose Isomerase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
FMV 35S promoter
0.67 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from SBVR111 vector pSYN15965
RZM gene originated from the Beet Necrotic Yellow Vein Virus (BNYVV). The gene RZM is the inverted repeat of a part of the RNA-1 gene transcript of the BNYVV. This portion of RNA-1 encodes the RNA dependent RNA polymerase (RdRp) or replicase protein. Expression of the RZM, driven by the promoter and intron from the Ubiquitin 3 (Ubi3) gene of Arabidopsis thaliana, confers resistance to BNYVV by targeting the replicase RNA transcript of the infecting virus via the RNAi mechanism.

DNA insert from H7-1 vector PV-BVGT08
H7-1 sugar beet was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered sugar beet line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows it to survive the otherwise lethal application of glyphosate. The EPSPS gene that was introduced into H7-1 was isolated from the common soil bacterium Agrobacterium tumefaciens, strain CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Risk Assessment2 records