| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
AVE-436G7-1 - Modena potato with altered starch
Record information and status
Record ID
101897
Status
Published
Date of creation
2011-06-15 09:27 UTC (hana_jirakova@env.cz)
Date of last update
2012-11-23 19:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-11-23 19:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Modena potato with altered starch
Transformation event
AV43-6-G7
Unique identifier
AVE-436G7-1
Developer(s)
BASF
BASF Plant Science GmbH
Carl-Bosch-Str. 64
Limburgerhof
Germany, 67117
Phone:+4962160-0
Fax:+4962160-28117
Email:securemail@basf.com
Description
Potato was modified by the insertion of a fragment of the granule-bound starch synthase gene (gbss) in the anti-sense direction to down-regulate the endogenous gbss gene.

The anti-sense repeat fragment of the gbss gene leads to a reduction of the amylose fraction in the starch of the potato tuber resulting in a concomitant increase in the levels of amylopectine.

The potato event AVE436G7-1 does not contain any selective genes for resistance to antibiotics or herbicides.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar: Karnico
Characteristics of the transformation process
Vector
pTiBo542ΔT
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
0.85 Kb
 
 
Granule-bound starch synthase gene
1.21 Kb
 
 
Granule-bound starch synthase gene
1.34 Kb
 
 
Granule-bound starch synthase gene
1.21 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A truncated portion of the coding sequence of gbss gene (CS-gbss) was cloned in anti-sense orientation under control of the gbss gene promoter (P-gbss).

AVE-436G7-1 contains one insert of an inverted repeat construct of the potato gbss gene. This LMO also contains part of the beta-galactosidase gene (lac) gene from Escherichia coli. No elements outside of the left-border (LB) and right-border (RB) of the T-DNA were inserted. Both LB and RB sequences of the T-DNA were truncated during the insertion.

The portions of the GBSS gene sequence in the construct organization in this LMO is as follows:
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, sense orientation in relation to the promoter sequence.
- GBSS cDNA fragment; 3' 1.3 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence, functioning as spacer element.
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Industrial
Detection method(s)
Additional information
Southern blot showed one insertion site with one copy of the constructed was inserted into AVE-436G7-1. The insertion site in the recipient organism (potato variety "Karnico") in which the T-DNA was integrated in AVE-436G7-1 was found to have a deletion of 1814 bp.
Additional Information

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism2 records
Organization1 record
Risk Assessment1 record