Modena potato with altered starch

Potato was modified by the insertion of a fragment of the granule-bound starch synthase gene (gbss) in the anti-sense direction to down-regulate the endogenous gbss gene.

The anti-sense repeat fragment of the gbss gene leads to a reduction of the amylose fraction in the starch of the potato tuber resulting in a concomitant increase in the levels of amylopectine.

The potato event AVE436G7-1 does not contain any selective genes for resistance to antibiotics or herbicides.

Cultivar: Karnico

pTiBo542ΔT

A truncated portion of the coding sequence of gbss gene (CS-gbss) was cloned in anti-sense orientation under control of the gbss gene promoter (P-gbss).

AVE-436G7-1 contains one insert of an inverted repeat construct of the potato gbss gene. This LMO also contains part of the beta-galactosidase gene (lac) gene from Escherichia coli. No elements outside of the left-border (LB) and right-border (RB) of the T-DNA were inserted. Both LB and RB sequences of the T-DNA were truncated during the insertion.

The portions of the GBSS gene sequence in the construct organization in this LMO is as follows:
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, sense orientation in relation to the promoter sequence.
- GBSS cDNA fragment; 3' 1.3 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence, functioning as spacer element.
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence.

Southern blot showed one insertion site with one copy of the constructed was inserted into AVE-436G7-1. The insertion site in the recipient organism (potato variety "Karnico") in which the T-DNA was integrated in AVE-436G7-1 was found to have a deletion of 1814 bp.