A truncated portion of the coding sequence of gbss gene (CS-gbss)
was cloned in anti-sense orientation under control of the gbss gene
promoter (P-gbss).
AVE-436G7-1 contains one insert of an inverted repeat construct of
the potato gbss gene. This LMO also contains part of the
beta-galactosidase gene (lac) gene from Escherichia coli. No
elements outside of the left-border (LB) and right-border (RB) of
the T-DNA were inserted. Both LB and RB sequences of the T-DNA were
truncated during the insertion.
The portions of the GBSS gene sequence in the construct
organization in this LMO is as follows:
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, sense
orientation in relation to the promoter sequence.
- GBSS cDNA fragment; 3' 1.3 kb part, Solanum tuberosum, antisense
orientation in relation to the promoter sequence, functioning as
spacer element.
- GBSS cDNA fragment; 5' 1.1 kb part, Solanum tuberosum, antisense
orientation in relation to the promoter sequence.
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