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Modified Organism
Pea modified for resistance to fungal diseases or insect pests
Record information and status
Record ID
101931
Status
Published
Date of creation
2011-06-16 13:28 UTC (hana_jirakova@env.cz)
Date of last update
2012-08-13 19:17 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-13 19:17 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Pea modified for resistance to fungal diseases or insect pests
Transformation event
pWell 09 and pWell11
Developer(s)
Agritec Plant Research
Agritec Plant Research
Zemedelska 2520/16
Sumperk
Czech Republic, 787 01
Phone:+420 583 382-111
Fax:+420 583 382-999
Email:info@agritec.cz
Url:Agritec Ltd.
Description
The transformation was mediated by the insertion of gmSPI-2 genes for protease inhibitors using Agrobacterium tumefaciens strain EHA105. pWell 09 and pWell 11 constructs contain a serine protease inhibitor gmSPI-2 gene.

The sequence was derived from cDNA of the protease inhibitor gene contained in wax moth´s (Galleria mellonella) silk glands. The supposed effect of the inhibitor consists in the inhibition of proteolytic enzymes in the insect´s digestive system, resulting in its starvation or death. The fungistatic and bacteriostatic effects of these genes have also been proved experimentally.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Pisum sativum - Garden pea, PEA
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Serine protease inhibitor gene - Galleria mellonella - Wax moth, Honeycomb Moth
Resistance to diseases and pests - Fungi
LMO characteristics
Modified traits
Additional Information
Other relevant website address or attached documents

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