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Modified Organism
Pea resistant to viral diseases
Record information and status
Record ID
101943
Status
Published
Date of creation
2011-06-17 05:44 UTC (hana_jirakova@env.cz)
Date of last update
2012-09-19 19:20 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-19 19:20 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Pea resistant to viral diseases
Transformation event
pWell05, pWell08, pWell07
Developer(s)
Agritec Plant Research
Agritec Plant Research
Zemedelska 2520/16
Sumperk
Czech Republic, 787 01
Phone:+420 583 382-111
Fax:+420 583 382-999
Email:info@agritec.cz
Url:Agritec Ltd.
Description
Pea plans were modifies for resistance to the viral pathogens Pea Enation Mosaic Virus (PEMV) or Pea Seed-borne Mosaic Virus (PSbMV).

The transformed genotypes contain selectable marker genes bar, conferring resistance to phosphinothricin - active component of Basta herbicide, and GUS for the facilitation of selection of genetically modified tissues and plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Pisum sativum - Garden pea, PEA
Characteristics of the transformation process
Vector
pWell05, pWell08 or pWell07
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
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CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
PSbMV coat protein
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PSbMV coat protein
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CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
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PEMV coat protein
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CaMV 35S terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The constructs of pWell05 and 08 lines contain a gene for cpPEMV coat protein.The pWell05 construct contains the whole sequence of the coat protein, whilst the pWell08 construct contains only a fragment of the sequence.

The pWell 07 construct contains a congenerous fragment of cpPSbMV gene for coat protein. The above-mentioned sequences should confer resistance to relevant viral pathogens (pea enation mosaic virus and pea seed-borne mosaic virus).
LMO characteristics
Modified traits
Common use(s)
  • Research

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