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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate tolerant maize
EN
VCO-Ø1981-5
Yes
VCO-Ø1981-5
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Person:Limagrain
63720, FrancePhone: +33 (0) 473-634 000,Fax:Email: limagrain@limagrain.com,Website: http://www.limagrain.com/,Related OrganizationGroupe Limagrain Holding S.A. ()Private sector (business and industry)Chappes,
63720, FrancePhone: +33 (0) 473-634 000,Fax:Email: limagrain@limagrain.com,Website: http://www.limagrain.com/,
Introduction of a tolerance to the glyphosate herbicide in maize plants by expression of a mutated form a bacterial 5-enolpyruvyl-shikimate-3-phosphate synthetase (EPSPS) protein insensitive to the herbicide. This herbicide acts as an inhibitor of this enzyme in plants. The EPSPS enzyme is involved in the biosynthetic pathway of the aromatic amino-acids (tryptophan, tyrosine and phenylalanine).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
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VCO-Ø1896-1 - Glyphosate tolerant maize| Groupe Limagrain Holding S.A. | Resistance to herbicides (Glyphosate)
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VCO-Ø19Ø2-7 - Glyphosate tolerant maize| Groupe Limagrain Holding S.A. | Resistance to herbicides (Glyphosate)
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VCO-Ø1936-5 - Glyphosate tolerant maize| Groupe Limagrain Holding S.A. | Resistance to herbicides (Glyphosate)
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VCO-Ø1853-3 - Glyphosate tolerant maize| Groupe Limagrain Holding S.A. | Resistance to herbicides (Glyphosate)
pAG3541
EN
- Agrobacterium-mediated DNA transfer
0.025 kb
|
1.800 kb
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0.198 kb
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1.242 kb
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0.270 kb
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0.025 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101941-3 Ubiquitin gene promoter | Saccharum sp. (Sugar cane)Promoter
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BCH-GENE-SCBD-101942-3 5-enolpyruvylshikimate-3-phosphate synthase | Arthrobacter globiformis (Arthrobacter)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-104660-4 Acetohydroxy acid synthase chloroplast transit peptide | Zea mays (Maize, Corn, MAIZE)Transit signal
Notes on the inserted genetic elements:
Ubiquitin promoter specifically obtained from Saccharum officinarum and includes the promoter region, 5'UTR and intron from the Ubiquitin 4 gene.
The mutagenesis of the original sequence of epsps gene from Arthobacter has been performed using the Mutazyme II system. Several mutations have been randomly performed, and modified epsps sequences have been inserted into E. coli. Subsequently, the activity of mutated genes has been tested by in vitro test based on enzymatic activity and thermal stability. The best sequence has been chosen to be used for the maize transformation.
Southern blot analysis indicated that a single intact insert was integrated into line VCO-Ø1981-5. No segments from the vector backbone were integrated into the LM maize.
EN
Ubiquitin promoter specifically obtained from Saccharum officinarum and includes the promoter region, 5'UTR and intron from the Ubiquitin 4 gene.
The mutagenesis of the original sequence of epsps gene from Arthobacter has been performed using the Mutazyme II system. Several mutations have been randomly performed, and modified epsps sequences have been inserted into E. coli. Subsequently, the activity of mutated genes has been tested by in vitro test based on enzymatic activity and thermal stability. The best sequence has been chosen to be used for the maize transformation.
Southern blot analysis indicated that a single intact insert was integrated into line VCO-Ø1981-5. No segments from the vector backbone were integrated into the LM maize.
EN
- Food
- Feed
- VCO-Ø1981-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
EN
EN
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