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Modified Organism
VCO-Ø1981-5 - Glyphosate tolerant maize
Record information and status
Record ID
101947
Status
Published
Date of creation
2011-06-17 12:26 UTC (hana_jirakova@env.cz)
Date of last update
2013-02-01 15:32 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-02-01 15:32 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Glyphosate tolerant maize
Transformation event
VCO-Ø1981-5
Unique identifier
VCO-Ø1981-5
Developer(s)
Limagrain
Groupe Limagrain Holding S.A.
Chappes
France, 63720
Phone:+33 (0) 473-634 000
Email:limagrain@limagrain.com
Url:Limagrain
Description
Introduction of a tolerance to the glyphosate herbicide in maize plants by expression of a mutated form a bacterial 5-enolpyruvyl-shikimate-3-phosphate synthetase (EPSPS) protein insensitive to the herbicide. This herbicide acts as an inhibitor of this enzyme in plants. The EPSPS enzyme is involved in the biosynthetic pathway of the aromatic amino-acids (tryptophan, tyrosine and phenylalanine).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
VCO-Ø1896-1 - Glyphosate tolerant maize
Limagrain Resistance to herbicides - Glyphosate
VCO-Ø19Ø2-7 - Glyphosate tolerant maize
Limagrain Resistance to herbicides - Glyphosate
VCO-Ø1936-5 - Glyphosate tolerant maize
Limagrain Resistance to herbicides - Glyphosate
VCO-Ø1853-3 - Glyphosate tolerant maize
Limagrain Resistance to herbicides - Glyphosate
Characteristics of the transformation process
Vector
pAG3541
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid left border repeat
0.03 Kb
 
 
Ubiquitin gene promoter
1.80 Kb
 
 
AHAS Chloroplast Transit Peptide
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.24 Kb
 
 
CaMV 35S terminator
0.27 Kb
 
 
Ti plasmid right border repeat
0.03 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Notes on the inserted genetic elements:
Ubiquitin promoter specifically obtained from Saccharum officinarum and includes the promoter region, 5'UTR and intron from the Ubiquitin 4 gene.

The mutagenesis of the original sequence of epsps gene from Arthobacter has been performed using the Mutazyme II system. Several mutations have been randomly performed, and modified epsps sequences have been inserted into E. coli. Subsequently, the activity of mutated genes has been tested by in vitro test based on enzymatic activity and thermal stability. The best sequence has been chosen to be used for the maize transformation.

Southern blot analysis indicated that a single intact insert was integrated into line VCO-Ø1981-5. No segments from the vector backbone were integrated into the LM maize.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed

Records referencing this document (13)
IDDescription
13record(s) found
Country's Decision or any other Communication4 records
Modified Organism4 records
Risk Assessment5 records