| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Tobacco transformed with fission yeast mitotic activator
Record information and status
Record ID
102014
Status
Published
Date of creation
2011-06-29 10:54 UTC (hana_jirakova@env.cz)
Date of last update
2012-08-13 18:58 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-13 18:58 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tobacco transformed with fission yeast mitotic activator
Transformation event
Transformation event unknown
Developer(s)
Dennis Francis
Cardiff University
Cardiff, Wales
United Kingdom of Great Britain and Northern Ireland, CF10 3XQ
Phone:+44 29208 76145
Email:FrancisD@cardiff.ac.uk
Description
Plants of Nicotiana tabacum were transformed with cdc25 gene from Schizosaccharomyces pombe under constitutive promotor 35S CaMV, together with an nptII gene for resistance to kanamycin. The transformation with the gene cdc25 significantly speeds up plant development and flowering earliness.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Nicotiana tabacum - Tobacco, TOBAC
Point of collection or acquisition of the recipient organism
Nicotiana tabacum L. cv. Samsun
Characteristics of the transformation process
Vector
pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.80 Kb
 
 
Cdc25 gene
1.74 Kb
 
 
Nopaline Synthase Gene Terminator
0.20 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Research
Detection method(s)
Additional information
- Polymerase chain reaction (PCR) with primers specific to inserted sequences
- Southern hybridization

Records referencing this document (1)
IDDescription
1record(s) found
Risk Assessment1 record