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Modified Organism
Potato modified for the expression of synthetic VP60 for vaccine production against RHDV
Record information and status
Record ID
102032
Status
Published
Date of creation
2011-07-07 14:00 UTC (german_bch@bvl.bund.de)
Date of last update
2013-02-08 14:59 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-02-08 14:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for the expression of synthetic VP60 for vaccine production against RHDV
Transformation event
Désirée 35SVP60SEK #17 #6
Developer(s)
Universität Rostock
Agrobiotechnology - Faculty of Agricultural and Environmental Sciences
Justus-von-Liebig-Weg 6

Rostock, Mecklenburg-Vorpommern
Germany, 18059
Phone:+49 381 498 ext 3080
Fax:+49 381 498 ext 3082
Email:dekan.auf@uni-rostock.de
Url:Rostock University,Agrobiotechnology - Rostock University
Description
Vaccines against rabbit hemorrhagic disease virus (RHDV) are derived from transgenic potato plants modified to express RHDV VP60. Rabbits immunized with leaf extracts from modified plants showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/ Variety: Désirée, Albatros
Characteristics of the transformation process
Vector
p35S (pLH9000-derivative)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Viral protein 60
0.00 Kb
 
 
SEKDEL ER retention signal
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
To enable expression of the VP60 gene in potatoes, a synthetic VP60 gene was introduced into potato plants via Agrobacterium tumefaciens-mediated gene transfer. This gene contains the sequence information of the VP60 gene from the RHDV virus isolate R-592, and codon-optimised using the tobacco genetic code. The synthetic gene fragment is 75% identical to the nucleotide sequence of VP60-gene and 100% identical to the amino acid sequence of the VP60 protein.

A DNA fragment that encodes the SEKDEL amino acids was added to the VP60 C-terminus sequence, thereby effecting the retention of the protein to the endoplasmic reticulum in the secretory pathway of protein synthesis. This is aimed at stabilising the protein.

In the plant, the expression is regulated by the 35S promoter from the cauliflower mosaic virus leading to expression in all plant parts throughout the entire lifecycle of the potato.
LMO characteristics
Modified traits
Common use(s)
  • Research
  • Vaccine

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record