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Modified Organism
Poplar with modified lignin
Record information and status
Record ID
102141
Status
Published
Date of creation
2011-08-05 13:14 UTC (didier.breyer@wiv-isp.be)
Date of last update
2012-08-28 18:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-28 18:43 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Poplar with modified lignin
Transformation event
WT52-3 and WT52-40
Developer(s)
Prof.dr. Wout Boerjan
Departement Plantensysteembiologie
VIB-UGent
Technologiepark 927
Gent
Belgium, B-9052
Phone:+32 9 331 38 81
Email:wout.boerjan@psb.vib-ugent.be
Url:Wout Boerjan Lab
Description
The genetically modified poplar exhibits modified lignin (a major constituent of wood) due to the decreased activity of an enzyme of the lignin biosynthetic pathway. The altered enzyme is CCR (Cinnamoyl coenzymeA reductase). The down-regulation has been obtained by co-suppression. The enzyme residual activity varies between 3 to 100 % and is not necessarily uniform within the plant. Consequently, the quality or/and quantity of lignin is modified.

These modifications and the consequences on some wood properties have been described in several publications (Baucher et al., 1996, van Doorsselaere et al., 1995 ; Meyermans et al., 2000 ; Lapierre et al., 1999 ; Pilate et al., 2002 ; Lapierre et al., 2004).

In addition, the transgenic lines have also integrated the hygromycin phosphotransferase (hpt) gene that confers resistance to the antibiotic hygromycin. This antibiotic resistance is needed during in vitro culture steps to select cells that have been genetically modified from those that have not been modified.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus alba - Gray Poplar
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: 717-1B4
Characteristics of the transformation process
Vector
pBIBHygro
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
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Cinnamoyl coenzymeA reductase
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CaMV 35S terminator
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CaMV Enhanced 35S promoter
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Cinnamoyl coenzymeA reductase
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Transcript 7 gene 3' untranslated region
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Nopaline Synthase Gene Promoter
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Hygromycin B phosphotransferase gene
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Transcript 7 gene 3' untranslated region
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The poplar ccr gene derives from cDNA sequences isolated from a xylem cDNA library from the Populus trichocarpa "Trichobel" clone. The full-length cDNA coding for CCR - Cinnamoyl Coenzyme A reductase (accession AJ224986 ; Leplé et al., 1998) is inserted in sense orientation between i) the promoter of the cauliflower mosaic virus (CaMV) in a duplicated version (a.k.a. p70) and ii) a terminator sequence, either from the T7 gene from the T-DNA (T7 terminator) or from the gene coding for the CaMV 35S RNA (T-35S).

The insertion of the ccr gene in the sense orientation aims at reducing the activity of the target enzyme, through a mechanism named co-suppression.

The resulting gene construct (p70-S-CCR-pA35S) once introduced in the pBIBHygro binary vector generates the pBIBHygro/SCCR pBIBHygro transformation vector.

The antibiotic resistance gene hygromycine B phosphotransferase (hpt or hph) was fused to the promoter of the nopaline synthase gene (P-nos) and to the terminator of the T7 gene of the Ti plasmid.

The inserted genetic material is the T-DNA from the Ti plasmid of Agrobacterium tumefaciens harbouring the constructs described above.
LMO characteristics
Modified traits
Common use(s)
  • Research
  • Biofuel
  • Fiber / Textile

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record