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Modified Organism
Potatoes modified for decreased susceptibility to Phytophthora infestans
Record information and status
Record ID
102168
Status
Published
Date of creation
2011-08-17 07:48 UTC (didier.breyer@wiv-isp.be)
Date of last update
2012-08-28 19:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-28 19:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potatoes modified for decreased susceptibility to Phytophthora infestans
Transformation event
Not available
Developer(s)
Prof. Dr. Godelieve Gheysen
Vakgroep Moleculaire Biotechnologie (Department Molecular Biology)
Universiteit Gent (UGent)
St-Pietersnieuwstraat 25
Gent
Belgium, BE-9000
Phone:+32-9-2645888
Email:godelieve.gheysen@ugent.be
Url:UGent- Molecular Biology Unit
Description
The genetically modified potatoes are less susceptible to late blight as a result of the introduction of one or three resistance genes stemming from wild relatives from the potato. All three resistance genes belong to the NBS-LRR class, which code for a class of proteins that are common in plants and are involved in disease resistance.

In addition, these potato lines contain the nptII gene for resistance against the antibiotic Kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
Agrobacterium Ti plasmid
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Phytophthora infestans Resistance gene 1 - Solanum stoloniferum - Wild potato
Resistance to diseases and pests - Fungi
Phytophthora infestans Resistance gene 1 - Solanum venturii - Wild Potato, SOLVN
Resistance to diseases and pests - Fungi
Phytophthora infestans Resistance gene 3 - Solanum bulbocastaneum - Nightshade, Ornamental nightshade
Resistance to diseases and pests - Fungi
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
Notes regarding the genetic elements introduced or modified in this LMO
The region to be inserted, which is flanked by the T-DNA borders from the Ti-plasmid of Agrobacterium tumefaciens contains either:

- One resistance gene (Rpi-vnt1, stemming from solanum venturii)

- One resistance gene and a antibiotic resistance gene as selection marker  (Rpi-sto1 + nptII, stemming from solanum stoloniferum and from Tn5 respectively)

- Three resistance genes and a selection marker gene (Rpi-vnt1 + Rpi-sto1 + Rpi-blb3 + nptII, stemming from solanum venturii)

All three Rpi-genes involved are under the control of their own promoters and terminators.

The npt-II gene expression is driven by the NOS promoter and terminator. The npt-II gene stems from the transposon Tn5. The NOS promoter and terminator originate from Agrobacterium tumefaciens.
LMO characteristics
Modified traits
  • Phytophthora infestans resistance
Common use(s)
  • Food
  • Feed

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record

   
   
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