Record details

Living Modified Organism identity

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LMO name

Potatoes modified for decreased susceptibility to Phytophthora infestans

Transformation event

Not available

Developer(s)

Record #102130

Prof. Dr. Godelieve Gheysen

Vakgroep Moleculaire Biotechnologie (Department Molecular Biology)

Universiteit Gent (UGent)

St-Pietersnieuwstraat 25

Gent

Belgium, BE-9000

Phone:	+32-9-2645888
Email:	godelieve.gheysen@ugent.be
Url:	UGent- Molecular Biology Unit

Description

The genetically modified potatoes are less susceptible to late blight as a result of the introduction of one or three resistance genes stemming from wild relatives from the potato. All three resistance genes belong to the NBS-LRR class, which code for a class of proteins that are common in plants and are involved in disease resistance.

In addition, these potato lines contain the nptII gene for resistance against the antibiotic Kanamycin.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12106

Solanum tuberosum - Potato, SOLTU

Characteristics of the transformation process

Vector

Agrobacterium Ti plasmid

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Introduced or modified genetic elements

Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.

Record #102164

Phytophthora infestans Resistance gene 1 - Solanum stoloniferum - Wild potato

Resistance to diseases and pests - Fungi

Record #102155

Phytophthora infestans Resistance gene 1 - Solanum venturii - Wild Potato, SOLVN

Resistance to diseases and pests - Fungi

Record #102165

Phytophthora infestans Resistance gene 3 - Solanum bulbocastaneum - Nightshade, Ornamental nightshade

Resistance to diseases and pests - Fungi

Record #100270

Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD

Record #15001

Neomycin Phosphotransferase II - Escherichia coli - ECOLX

Resistance to antibiotics - Kanamycin

Record #100269

Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD

Notes regarding the genetic elements introduced or modified in this LMO

The region to be inserted, which is flanked by the T-DNA borders from the Ti-plasmid of Agrobacterium tumefaciens contains either:

- One resistance gene (Rpi-vnt1, stemming from solanum venturii)

- One resistance gene and a antibiotic resistance gene as selection marker (Rpi-sto1 + nptII, stemming from solanum stoloniferum and from Tn5 respectively)

- Three resistance genes and a selection marker gene (Rpi-vnt1 + Rpi-sto1 + Rpi-blb3 + nptII, stemming from solanum venturii)

All three Rpi-genes involved are under the control of their own promoters and terminators.

The npt-II gene expression is driven by the NOS promoter and terminator. The npt-II gene stems from the transposon Tn5. The NOS promoter and terminator originate from Agrobacterium tumefaciens.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Fungi
Phytophthora infestans resistance

Common use(s)

Food
Feed