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Modified Organism
Petunia modified for antibiotic resistance
Record information and status
Record ID
102617
Status
Published
Date of creation
2011-10-27 08:38 UTC (german_bch@bvl.bund.de)
Date of last update
2015-10-21 16:37 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-21 16:37 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Petunia modified for antibiotic resistance
Transformation event
T16
Developer(s)
Universität Rostock
Agrobiotechnology - Faculty of Agricultural and Environmental Sciences
Justus-von-Liebig-Weg 6

Rostock, Mecklenburg-Vorpommern
Germany, 18059
Phone:+49 381 498 ext 3080
Fax:+49 381 498 ext 3082
Email:dekan.auf@uni-rostock.de
Url:Rostock University,Agrobiotechnology - Rostock University
Description
Petunia hybrida T16 are transplastomic plants that express the aadA and the uidA gene. The LMO was constructed for an evaluation of pollen mediated transfer rates of transgenes located in the plastid genome in field grown transgenic petunia.

While the aadA gene is used as a selection marker (resistance towards streptomycine and spectinomycine is conferred) the expression of the uidA gene in plastids enables visualisation of transgenic plastids. Furthermore the homologous recombination of the insert into the plastidial genome was mediated by the rbcL- and the accD-gene so that exclusively plastids were transformed but not the nuclear genome. Since pollen does not possess plastids a vertical gene transfers seems impractical.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Petunia hybrida - Petunia, PETHY
Characteristics of the transformation process
Vector
pUM73(AD)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
16S rRNA gene promoter
0.00 Kb
 
 
3"(9)-O-aminoglycoside adenyltransferase
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Photosystem II 44 kDa protein (psbC) terminator
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16S rRNA gene promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
D1 thylakoid membrane protein (psbA) gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The accD and the rbcL genes encompass the insert and enable homologous recombination into plastidial DNA of Petunia hybrida Juss. T16.

The uidA gene is used for the colorimetric and fluorimetric detection of transformed plastids.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research
Detection method(s)
Additional information
PCR-based methods need to be developed on information about the transformation vector.

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record