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Modified Organism
Wheat modified for increased protein content
Record information and status
Record ID
102891
Status
Published
Date of creation
2011-11-30 13:58 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-27 19:09 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-27 19:09 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Wheat modified for increased protein content
Transformation event
XAP Wheat
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
The endosperm-specific expression of the amino acid permease VfAAp1 in XAP wheat leads to increased amino acid uptake into the endosperm that might be used in the amino acid biosynthesis process. Therefore, an increase in the protein content in the wheat grains is expected. Additionally, wheat transformed with VfAAp1 is likely to exhibit early flowering.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Triticum aestivum - Wheat
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: Certo
Related LMOs
High-protein, herbicide resistant Barley HOSUT
Changes in physiology and/or production - Yield Changes in quality and/or metabolite content - Carbohydrates Resistance to herbicides - Glufosinate
Wheat modified for increased protein content
Changes in quality and/or metabolite content - Protein and amino acids Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
High-protein, antibiotic and herbicide resistant wheat SUTAP60
Changes in quality and/or metabolite content - Protein and amino acids Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
High-protein, antibiotic and herbicide resistant wheat SUTAP69
Changes in quality and/or metabolite content - Protein and amino acids Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
High-protein, antibiotic and herbicide resistant wheat SUTAP78
Changes in quality and/or metabolite content - Protein and amino acids Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
High-protein, herbicide resistant wheat HOSUT
Changes in physiology and/or production - Yield Changes in quality and/or metabolite content - Carbohydrates Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
pUC19/XAP and co-transformation of pJFBar
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
High molecular weight glutenin subunit 1Ax1 gene promoter
2.38 Kb
 
 
Amino acid permease 1 gene
1.43 Kb
 
 
Octopine Synthase Gene Terminator
0.73 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
By transformation of wheat with the vector pUC19/XAP the amino acid permease VfAAP1 was introduced into the wheat genome. Since VfAAP1 is put under the control of the endopserm-specific 1Ax 1 promoter and the octopine synthase terminator from Agrobacterium tumefaciens VfAAP1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains. This is meant to optimize the amino acid supply for increased levels of protein biosynthesis.

Also the integration of the beta lactamase gene from pUC19 was observed. This gene mediates resistance to bacteria towards ampicillin and was used as a selection marker prior to transformation of wheat plants. Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinat) to be used as a selective marker system.
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record