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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
High-protein, antibiotic and herbicide resistant wheat SUTAP60
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SUTAP60
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
The endosperm-specific expression of the amino acid permease VfAAp1 in SUTAP60 wheat leads to increased amino acid uptake into the endosperm that might be used in the amino acid biosynthesis process. Therefore, an increase in the protein content in the wheat grains is expected. Additionally, wheat transformed with VfAAp1 is likely to exhibit early flowering.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45396-4 Organism Triticum aestivum (Wheat)Crops
Cultivar/ Breeding line: Certo
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High-protein, antibiotic and herbicide resistant wheat SUTAP69| Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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High-protein, antibiotic and herbicide resistant wheat SUTAP78| Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
pUC18/SUTAP and pJFBar
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- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Ampicillin)
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BCH-GENE-SCBD-102882-3 Sucrose transporter promoter | Hordeum vulgare (Barley, HORVU)Promoter
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BCH-GENE-SCBD-48368-4 Amino acid permease 1 gene | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-103860-1 Amino acid permease 1 Terminator | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Terminator
By transformation of wheat with the vector pUC18/SUTAP the amino acid permease VfAAP1 was introduced into the wheat genome. Since VfAAP1 is put under the control of the HvSUT1 promoter it is specifically expressed within the transfer cells of the endosperm.
As a modification to the listed genetic element 'VfAAP1', here, the endogeneous terminator of VfAAP1 is joined to the coding sequence. VfAAP1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains. This is meant to optimize the amino acid supply for increased levels of protein biosynthesis.
Aditionally the integration of the beta lactamase gene from pUC19 was observed. This gene mediates resistance to bacteria towards ampicillin and was used as a selection marker prior to transformation of wheat plants.
Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinate) to be used as a selective marker system.
EN
As a modification to the listed genetic element 'VfAAP1', here, the endogeneous terminator of VfAAP1 is joined to the coding sequence. VfAAP1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains. This is meant to optimize the amino acid supply for increased levels of protein biosynthesis.
Aditionally the integration of the beta lactamase gene from pUC19 was observed. This gene mediates resistance to bacteria towards ampicillin and was used as a selection marker prior to transformation of wheat plants.
Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinate) to be used as a selective marker system.
EN
- Food
- Research
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