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Modified Organism
High-protein, herbicide resistant wheat HOSUT
Record information and status
Record ID
102894
Status
Published
Date of creation
2011-11-30 14:56 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-27 19:58 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-27 19:58 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
High-protein, herbicide resistant wheat HOSUT
Transformation event
HOSUT
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
The endosperm-specific expression of the sucrose transporter HvSUT1 in HOSUT wheat leads to increased sucrose uptake into the endosperm that might be used in the amino acid biosynthesis process. Therefore, an increase in the protein content in the wheat grains is expected.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Triticum aestivum - Wheat
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: Certo
Characteristics of the transformation process
Vector
pPZP 200 and pJFBar
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Hordein B1 promoter
0.00 Kb
 
 
Sucrose transporter gene
0.00 Kb
 
 
Hordein B1 terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
By transformation of wheat with the vector pPZP 200 the sucrose transporter HvSUT1 was introduced into the wheat genome. Since HvSUT1 is put under the control of the endopserm-specific hordein promoter B1 and the corresponding hordein terminator, HvSUT1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains.

This is meant to optimize the carbohydrate supply for increased levels of protein biosynthesis.

Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinate) to be used as a selective marker system.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Research

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record