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Modified Organism
Potato with altered starch composition
Record information and status
Record ID
102898
Status
Published
Date of creation
2011-12-08 10:22 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-27 18:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-27 18:40 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with altered starch composition
Transformation event
AM02, lines AM02-1003, AM02-1005, AM02-1008, AM02-1010, AM02-1012, AM02-1014 and AM02-1017
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
AM02 potatos synthesize starch that is practically amylose-free and primarily consists of amylopectin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
South America
Related LMOs
Potato with modified starch composition
BASF Plant Science GmbH altered carbohydrate composition: increased amylopectin content Resistance to antibiotics - Kanamycin Resistance to herbicides - Imidazolinone, Sulfonylurea
Potato with altered starch composition
altered carbohydrate composition: increased amylopectin content Resistance to antibiotics - Kanamycin
Potato modified for altered starch composition
altered carbohydrate composition: increased amylopectin content
Potato modified for altered starch content
BASF Plant Science GmbH altered carbohydrate composition: increased amylopectin content Resistance to herbicides - Imidazolinone, Sulfonylurea
Potato with altered starch content
BASF Plant Science GmbH altered carbohydrate composition: increased amylopectin content Resistance to herbicides - Imidazolinone, Sulfonylurea
Characteristics of the transformation process
Vector
pAP2
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
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Granule-bound starch synthase gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Acetohydroxy acid synthase gene
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Octopine Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Solanum tuberosum was transformed with a construct containing a fragment of the coding region of the potato granule bound starch synthase (gbss) gene in antisense direction. By introduction of an antisense-sequence the edogeneous gbss gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.

As a selection marker a mutated version of the gene ahas (acetohydroxy acid synthase (AHAS), acetolactate synthase (ALS)) from Arabidopsis thaliana was choosen. The expression of this gene is controlled by the nos promoter and the ocs terminator from Agrobacterium tumefaciens. The AHAS protein catalyzes the first step of the biosynthesis of the amino acids valine, leucine and isoleucine and a homologous gene can be found in all plants. Unlike the native gene the used mutated version of the AHAS protein cannot be inhibited by the herbicide Imazamox (active ingriedient: sulfonylurea and/or imidazolinone).  
LMO characteristics
Modified traits
Common use(s)
  • Research
Detection method(s)
Additional information
PCR based methods, to be developed on information about the transformation vector.

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment2 records