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Gene and DNA Sequence
Record information and status
Record ID
103022
Status
Published
Date of creation
2012-01-25 09:38 UTC (german_bch@bvl.bund.de)
Date of last update
2020-09-01 14:19 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-09-01 14:19 UTC (austein.mcloughlin@cbd.int)

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General information
Name of the gene or DNA sequence
Hexahistidine tag
Alternate Genetic Element Name (synonyms)
Hexa Histidine Anchor sequence
Abbreviation
CS-His-tag
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
Yes
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
Hexa Histidine Anchor
Biological function
The histidine affinity tag consists of 6 histidine amino acid residues located at the N- or C- terminus of recombinant protein. Purification of the recombination protein involves the use of immobilized metal affinity chromatography, which takes advantage of the high affinity of the hexahistidine residues for transition metal ions (e.g Ni2+ (most common), Zn2+, Cu2+ or Co2+).  Other proteins present in the lysate or solution are unlikely to bind to the immobilized metal ions, thus allowing for the isolation of  the recombinant protein. To release the recombinant protein from the resin containing the immobilized metal ions, the pH is lowered or a strong chelating agent (e.g. imidazole or EDTA) is used. Compared to other purification tags, histidine tags are small, have low toxicity and low levels of immunogenicity.
Related trait(s) or use(s) in biotechnology
  • Protein purification

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record