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Modified Organism
Garden Pea modified for the expression of a single chain antibody (scFv) in the seed
Record information and status
Record ID
103028
Status
Published
Date of creation
2012-01-25 09:43 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-16 19:34 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-16 19:34 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Garden Pea modified for the expression of a single chain antibody (scFv) in the seed
Transformation event
BA11
Developer(s)
Novoplant GmbH
Novoplant GmbH
Am Schwabeplan 1B
Gatersleben
Germany, 06466
Phone:+ 49 39482 7998-0
Fax:+ 49 39482 7998-80
Email:contact@novoplant.de
Description
BA11-2 pea plants express seed-specific, within the peas, the antibody fragment scFv BA11. This antibody fragment has affinity towards enterotoxigenic Escherichia coli (ETEC) cells that can cause the postweaning diarrhea by adhesion to the intestinal wall in pigs.

To prevent the outbreak of postweaning diarrhea BA11-expressing peas shell be feed to pigs.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Pisum sativum - Garden pea, PEA
Point of collection or acquisition of the recipient organism
Pisum sativum var. Eiffel
Characteristics of the transformation process
Vector
pPZP-USP(+)-BA11
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Unknown seed protein-promoter - Vicia faba - Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean
LeB4 Signal peptide - Vicia faba - Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean
Single-chain Fragment variable (scFv) antibody BA11 - Mus musculus - Mouse, House mouse, MOUSE
Production of medical or pharmaceutical compounds (human or animal) - Antibodies and antigens
KDEL ER retention signal
Hexa Histidine Anchor sequence
immunodetection and purification
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Notes regarding the genetic elements introduced or modified in this LMO
By action of the N-terminal signal peptide LeB4 and the C-terminal KDEL retetion signal the scFv BA11 antibody fragment accumulates within the endoplasmic reticulum but does not enter the secretion pathway. Since the LeB4-scFv BA11-KDEL fusion protein is under the control of the USP(+) promoter seed specific expression is achieved. A N-terminal His-tag allows immuno detection and immunoprecipitation of the fusion protein.
LMO characteristics
Modified traits
Common use(s)
  • Pharmaceutical
Detection method(s)
Additional information
PCR-based methods, to be developed on information about the transformation vector.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record