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Modified Organism
MON-8746Ø-4 - Droughtgard™ Maize
Record information and status
Record ID
103066
Status
Published
Date of creation
2012-02-06 18:59 UTC (bchrecords@inspection.gc.ca)
Date of last update
2013-07-02 18:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-02 18:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Droughtgard™ Maize
Transformation event
MON 87460
Unique identifier
MON-8746Ø-4
Developer(s)
Monsanto Canada Inc.
Description
MON 87460 was developed through Agrobacterium-mediated transformation of conventional maize variety embryos and expresses cold shock protein B (CspB) from Bacillus subtilis and NptII from Tn5 of Escherichia coli. MON 87460 was developed to provide reduced yield loss under water-limited conditions compared to conventional maize.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMAP595
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.36 Kb
 
 
Rice actin 1 gene promoter
0.92 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cold shock protein gene
0.20 Kb
 
 
Transcript 7 gene 3' untranslated region
0.51 Kb
 
 
loxP recombination site
0.03 Kb
 
 
CaMV 35S promoter
0.29 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
loxP recombination site
0.03 Kb
 
 
Ti plasmid left border repeat
0.44 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A disarmed Agrobacterium tumefaciens plant transformation system including the double-border, binary vector PV-ZMAP595 was used to transform LH59 embryos producing MON 87460.

The disarmed Agrobacterium tumefaciens transformation vector PV-ZMAP595 contains both left and right T-DNA border sequences facilitating the transformation. It was constructed using standard molecular biology techniques. The T-DNA region of this vector contains cspB and nptII expression cassettes and it is the portion that is integrated to maize genome during transformation event.

The genetic elements of PV-ZMAP595 intended for insertion into the maize genome comprised between the T-DNA borders are, from the right border region, promoter and leader from the rice actin gene (P-Ract1), a non-translated intron from the rice actin gene (I-Ract1), the cspB coding sequence (CS-cspB) and a polyadenylation sequence from the transcript 7 gene (T-tr7). These elements together constitute the cspB expression cassette which is followed by the nptII expression cassette. The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos).

MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. No additional elements from the transformation vector PV-ZMAP595, linked or unlinked to the cspB and nptII expression cassettes, were detected in the genome of MON 87460. Additionally, backbone sequence from the plasmid PV-ZMAP595 was not detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information
Other relevant website address or attached documents

Records referencing this document (26)
IDDescription
26record(s) found
Country's Decision or any other Communication10 records
Modified Organism4 records
Organization2 records
Risk Assessment10 records