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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato synthesizing cyanophycin biopolymer
EN
PsbY-cyel lines 12 and 23
No
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Organization:University of Rostock ()Academic or research institute, Agrobiotechnology - Faculty of Agricultural and Environmental SciencesFaculty of Agricultural and Environmental Sciences Justus-von-Liebig-Weg 6Rostock,
18059, GermanyPhone: +49 381 498 3001,Fax:Email: dekan.auf@uni-rostock.de,
The LMO PsbY-cyeI synthesizes the biopolymer cyanophycin which is a polyaspartate that finds broad application as a construction chemical and in the detergent industry. Through production of cyanophycin within potato plants a fully degradable substitute for petrochemical plastics should be provided.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Solanum tuberosum, variety Albatros
EN
derivate of pLH9000, pLH9000/PsbY-cyeI
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103097-4 PsbY transit peptide | Spinacia oleracea (Spinach, SPIOL)Transit signal
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BCH-GENE-SCBD-103096-2 Cyanophycin synthetase | Thermosynechococcus elongatus (Cyanobacteria)Protein coding sequence | Use in industrial applications
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
The LMO PsbY-cyeI expresses the cyanophicin synthetase cyeI as a fusion protein together with the transit peptide PsbY. Therefore the enzyme cyeI is translocated to the plastids of leaves and potato tubers of transgenic potato plants. The CaMV 35S promoter mediates an ubiquitious expression, the T-35S terminates the transcription of the fusion protein.
As a selection marker system the nptII gene was put under the control of the CaMV 35S promotor and -terminator. The gene product aminoglycosid 3´-phosphotransferase II catalyzes the phosphorylation of some aminoglycoside antibiotics, thereby inactivating these.
EN
As a selection marker system the nptII gene was put under the control of the CaMV 35S promotor and -terminator. The gene product aminoglycosid 3´-phosphotransferase II catalyzes the phosphorylation of some aminoglycoside antibiotics, thereby inactivating these.
EN
- Fiber/textile (production of a biopolymer)
PCR-based methods, to be developed on information about the transformation vector.
EN
EN
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