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Modified Organism
Potato synthesizing cyanophycin biopolymer
Record information and status
Record ID
103098
Status
Published
Date of creation
2012-02-20 13:48 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-16 19:53 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-16 19:53 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato synthesizing cyanophycin biopolymer
Transformation event
PsbY-cyel lines 12 and 23
Developer(s)
Universität Rostock
Agrobiotechnology - Faculty of Agricultural and Environmental Sciences
Justus-von-Liebig-Weg 6

Rostock, Mecklenburg-Vorpommern
Germany, 18059
Phone:+49 381 498 ext 3080
Fax:+49 381 498 ext 3082
Email:dekan.auf@uni-rostock.de
Url:Rostock University,Agrobiotechnology - Rostock University
Description
The LMO PsbY-cyeI synthesizes the biopolymer cyanophycin which is a polyaspartate that finds broad application as a construction chemical and in the detergent industry. Through production of cyanophycin within potato plants a fully degradable substitute for petrochemical plastics should be provided. 
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Solanum tuberosum, variety Albatros
Characteristics of the transformation process
Vector
derivate of pLH9000, pLH9000/PsbY-cyeI
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
PsbY transit peptide - Spinacia oleracea - Spinach, SPIOL
Cyanophycin synthetase - Thermosynechococcus elongatus - Cyanobacteria
Use in industrial applications
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Notes regarding the genetic elements introduced or modified in this LMO
The LMO PsbY-cyeI expresses the cyanophicin synthetase cyeI as a fusion protein together with the transit peptide PsbY. Therefore the enzyme cyeI is translocated to the plastids of leaves and potato tubers of transgenic potato plants. The CaMV 35S promoter mediates an ubiquitious expression, the T-35S terminates the transcription of the fusion protein.

As a selection marker system the nptII gene was put under the control of the CaMV 35S promotor and -terminator. The gene product aminoglycosid 3´-phosphotransferase II catalyzes the phosphorylation of some aminoglycoside antibiotics, thereby inactivating these. 
LMO characteristics
Modified traits
  • Use in industrial applications
Common use(s)
  • production of a biopolymer
Detection method(s)
Additional information
PCR-based methods, to be developed on information about the transformation vector.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record