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Modified Organism
Potato Modified for reduced expression of Zeaxanthin epoxidase
Record information and status
Record ID
103101
Status
Published
Date of creation
2012-02-20 13:58 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-16 20:18 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-16 20:18 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato Modified for reduced expression of Zeaxanthin epoxidase
Transformation event
Clone SR 47/00#18
Developer(s)
TU München
Lehrstuhl für Pflanzenzüchtung, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität München
Technische Universität München
Alte Akademie 8


Freising, Bayern
Germany, 85354
Phone:+49 8161 71 -3422,+49 8161 71 -3258
Fax:+49 8161 71-3900
Email:chris.schoen@wzw.tum.de,bork@wzw.tum.de,manuela.hochschulte@wzw.tum.de
Url:TU München, Lehrstuhl Pflanzenzüchtung,TU München - Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt
Description
A fragment of the coding sequence of Zeaxanthin epoxidase (zep) in antisense orientation is expressed in potato tubers. Thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers which leads to gene knock-down via the mechanisms of post-transcriptional gene silencing.

Reduced levels of zep mRNA cause missing synthesis of ZEP protein that is synonymous with an accumulation of zeaxanthin in potato tubers due to missing ZEP enzymatic activity. This may have an effect on carotinoid biosynthesis (also abscisic acid levels) and may impair the photoprotection during photosynthesis where ZEP is involved in the xanthophyll cycle. Phenotypically transgenic potato tubers can be distinguished by their altered colour compared to non-transgenic tubers.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line baltica
Related LMOs
Potato Modified for reduced expression of Zeaxanthin epoxidase
TU München Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to antibiotics - Kanamycin Tolerance to abiotic stress
Show detection method(s)
Characteristics of the transformation process
Vector
pPGBzep-antisense, derivate of pBIN19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
0.00 Kb
 
 
Zeaxanthin epoxidase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
LMO characteristics
Modified traits
  • accumulation of health improving secondary plant metabolites (zeaxanthin)
Other gene(s) whose expression was affected by the transformation
Zeaxanthin epoxidase gene - Solanum tuberosum - Potato, SOLTU
Changes in quality and/or metabolite content - Pigmentation / Coloration
Tolerance to abiotic stress
How the expression of the gene(s) was affected
RNA interference (RNAi) mediates gene silencing of endogeneous zep gene
Common use(s)
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information on the transformation vector

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record