Potato Modified for reduced expression of Zeaxanthin epoxidase | BCH-LMO-SCBD-103102 | Living Modified Organism | Biosafety Clearing-House

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BCH-LMO-SCBD-103102-3   |   PDF   |   Print   |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 20 Feb 2012 last updated: 17 Aug 2012
Living Modified Organism identity
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Potato Modified for reduced expression of Zeaxanthin epoxidase
EN
Clone SR 48/00#17
No
A fragment of the coding sequence of zep is expressed in potato tubers.  Thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers which thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers that leads to gene knock-down via sense suppression.

Reduced levels of zep mRNA cause missing synthesis of ZEP protein that is synonymous with an accumulation of zeaxanthin in potato tubers due to missing ZEP enzymatic activity. This may have an effect on carotenoid biosynthesis (also abscisic acid levels) and may impair the photoprotection during photosynthesis where ZEP is involved in the xanthophyll cycle. Phenotypically transgenic potato tubers can be distinguished by their altered colour compared to non-transgenic tubers.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line baltica
EN
  • Potato Modified for reduced expression of Zeaxanthin epoxidase
    | Technische Universität München | accumulation of health improving secondary plant metabolites (zeaxanthin), Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin)
Characteristics of the modification process
pPGBzep, derivate of pBIN19
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-100278-6 Zeaxanthin epoxidase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration),Tolerance to abiotic stress
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
EN
LMO characteristics
  • BCH-GENE-SCBD-100278-6 Zeaxanthin epoxidase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration),Tolerance to abiotic stress
By sense-suppression the endogeneous zep gene is silenced.
EN
  • Research
Detection method(s)
PCR-based methods, to be developed on information on the transformation vector.
EN
Additional Information
EN
Records referencing this document Show in search
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Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 1