Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato Modified for reduced expression of Zeaxanthin epoxidase
EN
Clone SR 48/00#17
No
-
Person:TU München, Lehrstuhl für Pflanzenzüchtung, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität MünchenAlte Akademie 8Freising, Bayern
85354, GermanyPhone: +49 8161 71 -3422, +49 8161 71 -3258,Fax: +49 8161 71-3900,Related OrganizationTechnische Universität München ()Academic or research instituteAlte Akademie 8Freising, Bayern
85354, GermanyPhone: +49 8161 71 -3422, +49 8161 71 -3258,Fax: +49 8161 71-3900,
A fragment of the coding sequence of zep is expressed in potato tubers. Thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers which thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers that leads to gene knock-down via sense suppression.
Reduced levels of zep mRNA cause missing synthesis of ZEP protein that is synonymous with an accumulation of zeaxanthin in potato tubers due to missing ZEP enzymatic activity. This may have an effect on carotenoid biosynthesis (also abscisic acid levels) and may impair the photoprotection during photosynthesis where ZEP is involved in the xanthophyll cycle. Phenotypically transgenic potato tubers can be distinguished by their altered colour compared to non-transgenic tubers.
EN
Reduced levels of zep mRNA cause missing synthesis of ZEP protein that is synonymous with an accumulation of zeaxanthin in potato tubers due to missing ZEP enzymatic activity. This may have an effect on carotenoid biosynthesis (also abscisic acid levels) and may impair the photoprotection during photosynthesis where ZEP is involved in the xanthophyll cycle. Phenotypically transgenic potato tubers can be distinguished by their altered colour compared to non-transgenic tubers.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/breeding line baltica
EN
-
Potato Modified for reduced expression of Zeaxanthin epoxidase| Technische Universität München | accumulation of health improving secondary plant metabolites (zeaxanthin), Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin)
pPGBzep, derivate of pBIN19
EN
- Agrobacterium-mediated DNA transfer
0.000 kb
|
0.000 kb
|
0.000 kb
|
0.000 kb
|
0.000 kb
|
0.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
-
BCH-GENE-SCBD-100278-6 Zeaxanthin epoxidase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration),Tolerance to abiotic stress
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
-
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
EN
-
BCH-GENE-SCBD-100278-6 Zeaxanthin epoxidase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration),Tolerance to abiotic stress
By sense-suppression the endogeneous zep gene is silenced.
EN
- Research
PCR-based methods, to be developed on information on the transformation vector.
EN
EN
Record type | Field | Record(s) | |||||
---|---|---|---|---|---|---|---|
|
|||||||
|
|||||||
|