Record details

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Living Modified Organism identity

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LMO name

Potato Modified for reduced expression of Zeaxanthin epoxidase

Transformation event

Clone SR 48/00#17

Developer(s)

TU München

Lehrstuhl für Pflanzenzüchtung, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität München

Technische Universität München

Alte Akademie 8

Freising, Bayern

Germany, 85354

Phone:	+49 8161 71 -3422,+49 8161 71 -3258
Fax:	+49 8161 71-3900
Email:	chris.schoen@wzw.tum.de,bork@wzw.tum.de,manuela.hochschulte@wzw.tum.de
Url:	TU München, Lehrstuhl Pflanzenzüchtung,TU München - Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt

Record #103100

Description

A fragment of the coding sequence of zep is expressed in potato tubers. Thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers which thereby mRNA homologous to the endogenous zep transcript is synthesized in potato tubers that leads to gene knock-down via sense suppression.

Reduced levels of zep mRNA cause missing synthesis of ZEP protein that is synonymous with an accumulation of zeaxanthin in potato tubers due to missing ZEP enzymatic activity. This may have an effect on carotenoid biosynthesis (also abscisic acid levels) and may impair the photoprotection during photosynthesis where ZEP is involved in the xanthophyll cycle. Phenotypically transgenic potato tubers can be distinguished by their altered colour compared to non-transgenic tubers.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Solanum tuberosum - Potato

Record #12106

Point of collection or acquisition of the recipient organism

Cultivar/breeding line baltica

Related LMOs

Potato Modified for reduced expression of Zeaxanthin epoxidase

TU München

Changes in quality and/or metabolite content - Pigmentation / Coloration
Resistance to antibiotics - Kanamycin
Tolerance to abiotic stress

Record #103101

Characteristics of the transformation process

Vector

pPGBzep, derivate of pBIN19

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Granule bound starch synthase gene promoter

#14997

0.00 Kb

Zeaxanthin epoxidase gene

#100278

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Nopaline Synthase Gene Promoter

#100270

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

LMO characteristics

Modified traits

Changes in quality and/or metabolite content
- Pigmentation / Coloration
Resistance to antibiotics
- Kanamycin
- Neomycin
accumulation of health improving secondary plant compounds (zeaxanthin)

Other gene(s) whose expression was affected by the transformation

Zeaxanthin epoxidase gene - Solanum tuberosum - Potato

Changes in quality and/or metabolite content - Pigmentation / Coloration
Tolerance to abiotic stress

Record #100278

How the expression of the gene(s) was affected

By sense-suppression the endogeneous zep gene is silenced.

Common use(s)

Research

Detection method(s)

Additional information

PCR-based methods, to be developed on information on the transformation vector.

Additional Information

Other relevant website address or attached documents

GeneticEngineering of a Zeaxanthin-richPotato by AntisenseInactivation and Co-suppression of CarotenoidEpoxidation