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Modified Organism
Potato producing vaccine against cholera
Record information and status
Record ID
103103
Status
Published
Date of creation
2012-02-20 13:50 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-22 15:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-22 15:30 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato producing vaccine against cholera
Transformation event
potato (Solanum tuberosum) 35SctxBSEK
Developer(s)
Universität Rostock
Agrobiotechnology - Faculty of Agricultural and Environmental Sciences
Justus-von-Liebig-Weg 6

Rostock, Mecklenburg-Vorpommern
Germany, 18059
Phone:+49 381 498 ext 3080
Fax:+49 381 498 ext 3082
Email:dekan.auf@uni-rostock.de
Url:Rostock University,Agrobiotechnology - Rostock University
Description
Expression of the non-toxic B subunit of the cholera toxin . The non-toxic B subunit has been used for more than 20 years as a component of human vaccines against cholera and other diarrheal diseases.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/ breeding line: desiree, albatros
Characteristics of the transformation process
Vector
p35SctxBSEK, derivative of pLH9000
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Cholera Toxin
0.00 Kb
 
 
SEKDEL ER retention signal
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The LMO 35SctxBSEK expresses a synthetic gene of the nontoxic subunit of cholera toxin that corresponds to 71% with the gene sequence of the ctxB gene from Vibrio cholerae (100% amino acid identity). The gene was adapted to the codon preference of higher plants. The ER-retention signal SEKDEL was fused to the cholera toxin subunit in order to stabilize the protein.
In the plant, expression is regulated by the 35S promoter and terminator from the cauliflower mosaic virus, which leads to an expression in all plant parts throughout the entire lifecycle of the potato.
LMO characteristics
Modified traits
Common use(s)
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on the information about the transformation vector.
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record