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Modified Organism
GTL-GFM311-6 - Cotton modified for insect resistance
Record information and status
Record ID
103111
Status
Published
Date of creation
2012-02-22 12:23 UTC (rainask@nathseeds.com)
Date of last update
2015-01-26 19:12 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 19:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cotton modified for insect resistance
Transformation event
GFM Cry1A
Unique identifier
GTL-GFM311-6
Developer(s)
Dr Satish Raina
Director
Global Transgenes Ltd (Nath Group Company)
Nath House, Nath Road, Aurangabad - 431005
Aurangabad
India
Phone:+91-240-2376568,+91-240-6645349
Fax:+91-240-2376188
Email:rainask@nathseeds.com,satishraina@yahoo.com
Description
This modified cotton contains the insecticidal cry1Ab/Ac gene which imparts resistance against Lepidoptera.

NOTE: This LMO was formerly referred to with the UID GTL-GFM311-7.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Characteristics of the transformation process
Vector
pGBI4AB derived from pBI121
Techniques used for the modification
  • Pollen Tube Pathway method (Chinese Patent)
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Cry1Ab/Ac - Bacillus thuringiensis - Bt, Bacillus, BACTU
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) - Cotton bollworm (Helicoverpa spp.)
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
This modified cotton contains the insecticidal cry1Ab/Ac gene which imparts resistance against Lepidoptera. The LMO also contains the selectable markers nptII to isolate transformed seedlings and a GUS gene cassette as a reporter gene.

Bt-cotton was generated by Pollen-Tube-Pathway mediated plasmid-DNA delivary into the embryo-sacs/egg apparatus. Following integration with the fertilized egg, the transformed embryos were detected by the nptII kanamycin selection system of the germinating seedlings. Putative transformants were analysed through Southerns and assayed for the level of insect resistance

The Bt transgene in the converted Indian inbred lines behaves as a single dominant Mendelian factor and is stably integrated in the plant genome.

The cry1Ab/Ac gene derived from Bacillus thuringiensis subsp. kurstaki (B.t.k.).
LMO characteristics
Modified traits
Common use(s)
  • Feed
Detection method(s)
Additional information
Immunological and DNA based detection methods have been developed for detection of cry1Ab-Ac protein. Central Institute of Cotton Research, Nagpur is the referral laboratory for the same.
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record