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Gene and DNA Sequence
Record information and status
Record ID
103123
Status
Published
Date of creation
2012-02-24 12:44 UTC (german_bch@bvl.bund.de)
Date of last update
2012-07-26 13:39 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-07-26 13:39 UTC (dina.abdelhakim@cbd.int)

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General information
Name of the gene or DNA sequence
Pyruvate orthophosphate dikinase, Intron 3
Abbreviation
I-pdk i3
Category of DNA sequence
  • Intron
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Flaveria trinervia - Clustered Yellowtops, speedyweed, flaveria, yellow twinstem
Additional Information
Additional Information
pdk i3 is used as a spacer sequence between antisense- and sense gene fragments in RNAi constructs to enhance vector stability. When expressed, the intron is spliced and the remaining RNA forms an inverted repeat double stranded structure triggering RNA silencing of the respective target gene. Pdk i3 was cloned into standard vectors used for gene silencing approaches such as pHELLSGATE.

The full gene sequence that includes intron 3 of the pyruvate orthophosphate dikinase (pdk i3) codes for the PPDK enzyme. PPDK is a key enzyme of the C4-photosynthesis mechanism. Its catalysis product phosphoenol pyruvate acts as an acceptor molecule for the primary CO2-fixation in mesophyll cells during C4-photosynthesis. Also in C3-plants PPDK isoforms are known where they most likely fulfil an anaplerotic function by providing carbon skeletons for amino acid biosynthesis.

Records referencing this document (23)
IDDescription
23record(s) found
Country's Decision or any other Communication7 records
Modified Organism9 records
Risk Assessment7 records