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Modified Organism
Potato modified for heat tolerance and increased yield
Record information and status
Record ID
103124
Status
Published
Date of creation
2012-02-24 12:45 UTC (german_bch@bvl.bund.de)
Date of last update
2013-03-20 13:54 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-20 13:54 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for heat tolerance and increased yield
Transformation event
StSDDhpi - 4 independant lines #1, #23, #41 and #45
Developer(s)
Max Planck Institute of Molecular Plant Physiology (MPIMP)
Max-Planck-Institut für Molekulare Pflanzenphysiologie
Am Mühlenberg 1
Potsdam
Germany, 14476
Phone:+49 331 567 - 80
Fax:+49 331 567 - 84 08
Email:contact@mpimp-golm.mpg.de
Url:Max-Planck-Institut für Molekulare Pflanzenphysiologie
Description
Transgenic potatoes StSDDhpi exhibit an increase in stomata density. Most probably this has an effect on gas and water exchange of the plants with the atmosphere. The genetically modified plants displayed increased heat tolerance and since this influences photosynthetic activity the increase in stomata density is thought to also increases the yield of potato tubers. To achieve this increase in stomata density the gene stomatal density and distribution 1 (sdd1) was silenced via RNAi since a sdd1 knock-out mutant of Arabidopsis thaliana demonstrated that sdd1 is a negative  regulator of stomata development.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Related LMOs
Potato modified for increased Stomata Density
Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Drought
Potato modified for decreased Stomata Density
Resistance to antibiotics - Hygromycin Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
pSTSDDhpi, derivate of the plant transformation vector pART27
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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Stomatal density and distribution 1 gene
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Pyruvate orthophosphate dikinase, Intron 3
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Stomatal density and distribution 1 gene
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Octopine Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The inserted T-DNA consists of several cassettes: First, a RNAi construct that is build of a StSDD1 gene fragment in sense- and antisense orientation separated by the pdk intron 3 promotes the generation of double stranded RNA (dsRNA) in a hairpin conformation. This dsRNA targets the endogenous SDD1 transcript and therefore mediates post transcriptional gene silencing. The RNAi construct is controlled by the CaMV 35S promoter and the ocs terminator.

Second, as a selection marker system nptII gene is used whose gene product aminoglycoside 3-phosphotransferase II confers resistance to aminoglycoside antibiotics (kanamycin, neomycin, gentenicin). NptII is controlled by the nos promoter and -terminator.

Furthermore, sequence fragments of the lacI gene, the lacZ promoter and the lacZ gene of Escherichia coli are encoded by the inserted DNA but are not functional in plants.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Stomatal density and distribution 1 gene - Solanum tuberosum - Potato, SOLTU
Tolerance to abiotic stress - Drought
How the expression of the gene(s) was affected
By a RNAi construct the expression of the endogeneous SDD1 gene is down regulated.
Common use(s)
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information on the transformation vector pSTSDDhpi.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record