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Biosafety Information Resource
Record information and status
Record ID
103411
Status
Published
Date of creation
2012-05-09 16:06 UTC (dina.abdelhakim@cbd.int)
Date of last update
2012-05-14 19:29 UTC (dina.abdelhakim@cbd.int)

General Information
Title
Proteomic analysis of a genetically modified maize flour carrying CRY1AB gene and comparison to the corresponding wild-type
Author
A. Giuliano Albo, S. Mila, G. Digilio, M. Motto, S. Aime, D. Corpillo
Author’s contact information
D. Corpillo
Email: proteomics@bioindustrypark.it

Laboratorio Integrato Metodologie Avanzate,
Bioindustry Park Canavese,
Via Ribes 5, 10010 Colleretto Giacosa, Italy

fax: +39 0125 53028
Language(s)
  • English
Publication date
2007
Subject
Summary, abstract or table of contents
Abstract:

Protein expression in a maize hybrid flour (hereafter called WT) and its corresponding transgenic version resistant to European corn borer (hereafter called BT and carrying a gene encoding for the Bacillus thuringiensis insecticidal protein Cry1Ab) has been studied by means of two-dimensional gel electrophoresis coupled with mass spectrometry. This comparison has been chosen as a model to verify proteomics capability in detecting unexpected differences between near-isogenic lines (differing in the ideal case only for the presence of the transgenic protein). Some unpredictable differences were detected: i) glucose and ribitol dehydrogenase spot was unique of BT maize; ii) endochitinase A spot was unique of WT maize; iii) triosephosphate isomerase 1 and one spot of globulin-1 S were overexpressed while cytosolic 3-phosphoglycerate kinase and one spot of aldose reductase were down-regulated in BT maize with respect to WT. These results outline the potential of the new nontargeted "-omics" technologies (in particular proteomics) in the detection of unexpected, unintended and unwanted variations in Genetically Modified (GM) versus non-GM food comparison and suggest the possible employment of these technologies in substantial equivalence evaluation. Moreover, a reference map for maize flour was built: forty spots, corresponding to twenty-five different proteins, were successfully identified.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Yes
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
ISSN: 00256153
Publisher and its location
Maydica
Bergamo, Italy
Format
14 page PDF
Source
Maydica
Keywords and any other relevant information
Keywords: Cry1Ab; Genetically modified plants; Maize; Proteomics; Substantial equivalence.

Citation:Maydica 52 (2007): 443-455

   
   
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