Record details

General Information

Title

Proteomic analysis of a genetically modified maize flour carrying CRY1AB gene and comparison to the corresponding wild-type

Author

A. Giuliano Albo, S. Mila, G. Digilio, M. Motto, S. Aime, D. Corpillo

Author’s contact information

D. Corpillo
Email: proteomics@bioindustrypark.it

Laboratorio Integrato Metodologie Avanzate,
Bioindustry Park Canavese,
Via Ribes 5, 10010 Colleretto Giacosa, Italy

fax: +39 0125 53028

Language(s)

English

Publication date

2007

Subject

Summary, abstract or table of contents

Abstract:

Protein expression in a maize hybrid flour (hereafter called WT) and its corresponding transgenic version resistant to European corn borer (hereafter called BT and carrying a gene encoding for the Bacillus thuringiensis insecticidal protein Cry1Ab) has been studied by means of two-dimensional gel electrophoresis coupled with mass spectrometry. This comparison has been chosen as a model to verify proteomics capability in detecting unexpected differences between near-isogenic lines (differing in the ideal case only for the presence of the transgenic protein). Some unpredictable differences were detected: i) glucose and ribitol dehydrogenase spot was unique of BT maize; ii) endochitinase A spot was unique of WT maize; iii) triosephosphate isomerase 1 and one spot of globulin-1 S were overexpressed while cytosolic 3-phosphoglycerate kinase and one spot of aldose reductase were down-regulated in BT maize with respect to WT. These results outline the potential of the new nontargeted "-omics" technologies (in particular proteomics) in the detection of unexpected, unintended and unwanted variations in Genetically Modified (GM) versus non-GM food comparison and suggest the possible employment of these technologies in substantial equivalence evaluation. Moreover, a reference map for maize flour was built: forty spots, corresponding to twenty-five different proteins, were successfully identified.

Thematic areas

Scientific and technical issues
- Risk assessment

Background material to the “Guidance on risk assessment of living modified organisms”

Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”

http://bch.cbd.int/onlineconferences/guidance_ra

Yes

Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant

Part I: Roadmap for risk assessment of living modified organisms
- Conducting the risk assessment
  - Step 1: “an identification of any novel genotypic and phenotypic characteristics associated with the living modified organism that may have adverse effects on biological diversity in the likely potential receiving environment, taking also into account risks to human health”

Additional Information

Type of resource

Article (journal / magazine / newspaper)

Identifier

ISSN: 00256153

Publisher and its location

Maydica
Bergamo, Italy

Format

14 page PDF

Source

Maydica

Keywords and any other relevant information

Keywords: Cry1Ab; Genetically modified plants; Maize; Proteomics; Substantial equivalence.

Citation:Maydica 52 (2007): 443-455

Access to the resource

Link to the resource(s)

Proteomic analysis of a genetically modified maize flour carrying CRY1AB gene and comparison to the corresponding wild-type.pdf

Maydica: Proteomic analysis of a genetically modified maize flour carrying CRY1AB gene and comparison to the corresponding wild-type