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Record information and status
Record ID
103428
Status
Published
Date of creation
2012-05-10 15:36 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-10 15:36 UTC (dina.abdelhakim@cbd.int)

General Information
Title
Transcriptome and metabolome profiling of fieldgrown transgenic barley lack induced differences but show cultivar-specific variances
Author
Karl-Heinz Kogel, Lars M. Voll, Patrick Schäfer, Carin Jansena, Yongchun Wuc, Gregor Langen, Jafargholi Imani, Jörg Hofmann, Alfred Schmiedl, Sophia Sonnewald, Diter von Wettsteina, R. James Cook, and Uwe Sonnewald
Author’s contact information
Diter von Wettstein or Uwe Sonnewald
E-mail: diter@wsu.edu or usonne@biologie.uni-erlangen.de.
Language(s)
  • English
Publication date
2010-04-06
Subject
Summary, abstract or table of contents
Abstract:

The aim of the present study was to assess possible adverse effects of transgene expression in leaves of field-grown barley relative to the influence of genetic background and the effect of plant interaction with arbuscular mycorrhizal fungi. We conducted transcript profiling, metabolome profiling, and metabolic fingerprinting of wild-type accessions and barley transgenics with seed-specific expression of (1,3-1, 4)-β-glucanase (GluB) in Baronesse (B) as well as of transgenics in Golden Promise (GP) background with ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). We found more than 1,600 differential transcripts between varieties GP and B, with defense genes being strongly overrepresented in B, indicating a divergent response to subclinical pathogen challenge in the field. In contrast, no statistically significant differences between ChGP and GP could be detected based on transcriptome or metabolome analysis, although 22 genes and 4 metabolites were differentially abundant when comparing GluB and B, leading to the distinction of these two genotypes in principle component analysis. The coregulation of most of these genes in GluB and GP, as well as simple sequence repeat-marker analysis, suggests that the distinctive alleles in GluB are inherited from GP. Thus, the effect of the two investigated transgenes on the global transcript profile is substantially lower than the effect of a minor number of alleles that differ as a consequence of crop breeding. Exposing roots to the spores of the mycorrhizal Glomus sp. had little effect on the leaf transcriptome, but central leaf metabolism was consistently altered in all genotypes.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Yes
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
doi: 10.1073/pnas.1001945107
Publisher and its location
National Academy of Sciences of the United states of America
New York
Rights
Open Access
Format
6 page PDF
Source
Proceedings of the National Academy of Sciences (PNAS)
Keywords and any other relevant information
Keywords: food safety, glucanase, chitinase, sustainability

Citation: PNAS April 6, 2010 vol. 107 no. 14 6198-6203