| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Maize with altered growth and tolerance to herbicides
Record information and status
Record ID
103523
Status
Published
Date of creation
2012-04-24 11:18 UTC (didier.breyer@wiv-isp.be)
Date of last update
2019-08-20 18:05 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-20 18:05 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize with altered growth and tolerance to herbicides
Transformation event
pUBIL::GA20OX1
Developer(s)
Prof. Dr. Frank Van Breusegem
Departement Planten Systeembiologie
VIB-UGent
Technologiepark 927
Gent
Belgium, 9052
Phone:+ 32 (0)9 33 13 920
Email:frbre@psb.vib-ugent.be
Url:VIB
Description
The genetically modified maize has an altered growth characteristic resulting from the introduction of a Gibberelin 20 Oxidase-1 gene from Arabidopsis thaliana to increase levels of gibberellic acid. The plants become significantly longer and more slender without producing significantly more biomass. At the cellular level, the plants produced more cellulose, lignin, and cell wall residue.

Additionally the bar-gene from Streptomyces hygroscopicus is present as a selectable marker gene. The gene encodes phosphinotricin acetyl transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Zea mays breeding line: inbred line B104
Characteristics of the transformation process
Vector
pMBb7Fm21GW-UBIL
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S terminator
0.23 Kb
 
 
Gibberellin 2-beta-dioxygenase gene
1.13 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Omega 5' untranslated leader
0.09 Kb
 
 
CaMV 35S promoter
0.94 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Two gene cassettes are present in the modified maize:

I. GA20oxidase
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.

II. bar
Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.

LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (3)
IDDescription
3record(s) found
Modified Organism2 records
Risk Assessment1 record