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Modified Organism
Potato modified for the production of spider silk protein
Record information and status
Record ID
103555
Status
Published
Date of creation
2012-05-30 15:15 UTC (german_bch@bvl.bund.de)
Date of last update
2012-08-24 14:31 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-24 14:31 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for the production of spider silk protein
Transformation event
Solanum tuberosum pCB301-Kan-MaSpII-100xELP - 3 lines: 26, 27, 28
Developer(s)
Institut für Pflanzengenetik und Kulturpflanzenforschung
Corrensstrasse 3 
Gatersleben
Germany, 06466
Phone:+49 (0)39482 5-0
Fax:+49 (0) 39482 5139
Email:info@ipk-gatersleben.de
Url:IPK Gatersleben
Description
Potatoes pCB301-Kan-MaSpII-100xELP produce a fusion protein consisting of the spider silk protein MaSPII and an artificial elastin which is mainly observed in tubers.

Female golden silk orbweaver spiders Nephila clavipes incorporate MaSpII protein within the very strong and elastic silk of the main threads of the spider net which is of high industrial interest.

The artificial elastin 100xELP has high similarity to human elastin and consists of oligomeric repeats of the pentapaptide Val-Pro-Gly-Xaa-Gly (Xaa - any amino acid but proline). 100xELP exhibits a good ability to be solved in aqueous solution depending on salt concentration and temperature. It can undergo an inverse transition, i.e. the protein is soluble in an aqueous solution below its transition temperature but insoluble above this critical value.

This characteristic is delegated to its fusion partner MaSpII resulting in easy isolation and purification of spider silk protein from the biomass of potato tubers.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Albatros
Related LMOs
Potato modified to synthesise fusion spider silk/ elastin protein
Production of medical or pharmaceutical compounds (human or animal) Resistance to antibiotics - Kanamycin Use in industrial applications
Potato modified to synthesise fusion spider silk fragment/ elastin protein
Production of medical or pharmaceutical compounds (human or animal) Resistance to antibiotics - Kanamycin Use in industrial applications
Characteristics of the transformation process
Vector
pCB301-Kan-MaSpII-100xELP, a derivat of the binary vector pCB301-Kan
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
LeB4 Signal peptide
0.00 Kb
 
 
Major Spidroin II protein coding sequence
0.00 Kb
 
 
c-myc-tag
0.00 Kb
 
 
Synthetic elastin-like protein
0.00 Kb
 
 
KDEL ER retention signal
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Part of the multiple cloning site of the plasmid pBluescriptII and approximately 160 nucleotides of the right and 140 nucleotides of the left boarder sequence of the T-DNA are inserted in the potato genome but not functional in plants.
LMO characteristics
Modified traits
  • Production of medical or pharmaceutical compounds (human or animal)
  • Use in industrial applications
Common use(s)
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information about the transformation vector.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record