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Modified Organism
Cowpea resistant to lepidoptera pests
Record information and status
Record ID
103630
Status
Published
Date of creation
2012-06-18 17:56 UTC (manoela.miranda@cbd.int)
Date of last update
2019-02-27 15:50 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-02-27 15:50 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cowpea resistant to lepidoptera pests
Transformation event
Twelve lines: 709A, 710B, 711B2, 708A, 808B1, 162B2, 716F, 717B, 715A, 152D, 155A, 720D
Developer(s)
AATF
The African Agricultural Technology Foundation (AATF)
P.O. Box 30709
Nairobi
Kenya, 00100
Phone:+ 254-20 422 3700
Email:aatf@aatf-africa.org
Url:The African Agricultural Technology Foundation
Description
Bt-cowpea is genetically modified by insertion of gene Cry1Ab. It is resistant to Maruca vitrata Fab. (pod borer), one of the main depredators of cultivated and wild cowpea.

Bt-cowpea was genetically modified through a transformation mediated by Agrobacterium. It expresses the Cry1Ab protein, which is present in the tissues of the plant and confers resistance to pod borer.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Vigna unguiculata - Cowpea, Black eyed pea
Related LMOs
AAT-7Ø9AA-4 - Pod Borer-resistant cowpea
AATF and Institute of Agricultural Research Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
pMB4 derived from pArt27
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
SCSV1 promoter
0.53 Kb
 
 
Neomycin Phosphotransferase II
0.18 Kb
 
 
Catalase 1 intron
0.19 Kb
 
 
Neomycin Phosphotransferase II
0.62 Kb
 
 
SCSV3 terminator
0.14 Kb
 
 
rbcS Promoter
1.72 Kb
 
 
Cry1Ab
1.87 Kb
 
 
rbcS Terminator
0.49 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The sequence of the Cry1Ab is  derived from the kurstaki HD1 lineage of Bacillus thuringiensis. The gene is modified for optimal expression in plants by removing a polyadenelation signal, increasing the AT content and removing destabilising sequences.

The NptII gene (971 bp) is under the control of an unknown promoter (531bp) and terminator (138 bp) originating from Subterranean clover stunt virus. The expression cassette also contains an intron from the Catalase I gene from Ricinus communis which reduces the expression of the gene in bacteria.

The binary gene construct consists of the  Cry2Ab coding region with a chloroplast targeting peptide (MBE) and selective marker gene nptII conferring resistance to the antibiotic kanamycin. Modification was by Agrobacterium tumifaciens.
 
Note: Southern Blot analysis indicated a single T-DNA insertion.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Research

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication2 records
Modified Organism1 record
Risk Assessment2 records