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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Cowpea resistant to lepidoptera pests
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Twelve lines: 709A, 710B, 711B2, 708A, 808B1, 162B2, 716F, 717B, 715A, 152D, 155A, 720D
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Person:AATFP.O. Box 30709Nairobi,
00100, KenyaPhone: + 254-20 422 3700,Fax:Email: aatf@aatf-africa.org,Website: http://www.aatf-africa.org/en/,Related OrganizationThe African Agricultural Technology Foundation (AATF) ()Non-governmental organization (NGO)P.O. Box 30709Nairobi,
00100, KenyaPhone: + 254-20 422 3700,Fax:Email: aatf@aatf-africa.org,Website: http://www.aatf-africa.org/en/,
Bt-cowpea is genetically modified by insertion of gene Cry1Ab. It is resistant to Maruca vitrata Fab. (pod borer), one of the main depredators of cultivated and wild cowpea.
Bt-cowpea was genetically modified through a transformation mediated by Agrobacterium. It expresses the Cry1Ab protein, which is present in the tissues of the plant and confers resistance to pod borer.
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Bt-cowpea was genetically modified through a transformation mediated by Agrobacterium. It expresses the Cry1Ab protein, which is present in the tissues of the plant and confers resistance to pod borer.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-103617-3 Organism Vigna unguiculata (Cowpea, Black eyed pea)Crops
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AAT-7Ø9AA-4 - Pod Borer-resistant cowpea| African Agricultural Technology Foundation and Institute of Agricultural Research | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
pMB4 derived from pArt27
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- Agrobacterium-mediated DNA transfer
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0.530 kb
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0.180 kb
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0.190 kb
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0.620 kb
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0.140 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-103853-2 rbcS Terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-114438-2 SCSV1 promoter | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Promoter
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BCH-GENE-SCBD-114274-2 Catalase 1 intron | Ricinus communis (Castor bean)Intron
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BCH-GENE-SCBD-114440-1 SCSV3 terminator | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Terminator
The sequence of the Cry1Ab is derived from the kurstaki HD1 lineage of Bacillus thuringiensis. The gene is modified for optimal expression in plants by removing a polyadenelation signal, increasing the AT content and removing destabilising sequences.
The NptII gene (971 bp) is under the control of an unknown promoter (531bp) and terminator (138 bp) originating from Subterranean clover stunt virus. The expression cassette also contains an intron from the Catalase I gene from Ricinus communis which reduces the expression of the gene in bacteria.
The binary gene construct consists of the Cry2Ab coding region with a chloroplast targeting peptide (MBE) and selective marker gene nptII conferring resistance to the antibiotic kanamycin. Modification was by Agrobacterium tumifaciens.
Note: Southern Blot analysis indicated a single T-DNA insertion.
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The NptII gene (971 bp) is under the control of an unknown promoter (531bp) and terminator (138 bp) originating from Subterranean clover stunt virus. The expression cassette also contains an intron from the Catalase I gene from Ricinus communis which reduces the expression of the gene in bacteria.
The binary gene construct consists of the Cry2Ab coding region with a chloroplast targeting peptide (MBE) and selective marker gene nptII conferring resistance to the antibiotic kanamycin. Modification was by Agrobacterium tumifaciens.
Note: Southern Blot analysis indicated a single T-DNA insertion.
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- Food
- Feed
- Research
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