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Modified Organism
Potato with altered starch quality
Record information and status
Record ID
103636
Status
Published
Date of creation
2012-06-19 20:37 UTC (manoela.miranda@cbd.int)
Date of last update
2012-06-28 14:49 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with altered starch quality
Transformation event
pMFIp9-1, line #1332
Developer(s)
LfL Freising
Institut für Pflanzenbau und Pflanenzüchtung
Bayerische Landesanstalt für Landwirtschaft
Vöttinger Strasse 38

Freising, Bayern
Germany, 85354
Phone:+ 49 8161 71-5800
Fax:+ 49 8161 71-5809
Email:Poststelle@LfL.bayern.de
Url:http://www.lfl.bayern.de/ipz/biotechnologie/33232/index.php
Description
Solanum tuberosum was transformed with a construct containing a 581 bp long fragment of exon 2 of the potato granule bound starch synthase (GBSS) gene in antisense orientation. The transcription of this fragment is controlled by its native, tuber-specific promoter P-gbss and its contiguous non-translated leader sequence - consisting of exon 1 and intron 1 of the GBSS gene. The transcription is terminated by the CaMV 35S terminator from Cauliflower mosaic virus.

The markergen-free amylopectin potato pMFIp9-1, line #1332 exhibits an altered starch quality compared to wild-type potato. Nearly pure amylopectin-starch is synthesized.

By introduction of an antisense-sequence the endogenous GBSS gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.

This transgenic organism does not contain any marker genes for selection purposes within the inserted T-DNA.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato
Point of collection or acquisition of the recipient organism
Solanum tuberosum L. of cultivar Walli transformed
Related LMOs
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AVE-436G7-1 - Modena potato with altered starch
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Selectable marker genes and reporter genes
Potato with altered starch composition
BASF Plant Science GmbH

altered carbohydrate composition: increased amylopectin content
Resistance to herbicides - Imidazolinone, Sulfonylurea
Characteristics of the transformation process
Vector
pMFIp9-1
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
0.00 Kb
 
 
Granule-bound starch synthase gene
581.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Granule-bound starch synthase gene - Solanum tuberosum - Potato
altered carbohydrate composition: increased amylopectin content
How the expression of the gene(s) was affected
The GBSS gene is silenced post-transcriptionally.
Common use(s)
  • Food
  • Feed
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information on the transformation vector.
Additional Information

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record

   
   
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