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Living Modified Organism (LMO)
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Potato with altered starch quality
EN
pMFIp9-1, line #1332
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Person:LfL Freising, Institut für Pflanzenbau und PflanenzüchtungVöttinger Strasse 38Freising, Bayern
85354, GermanyPhone: + 49 8161 71-5800,Fax: + 49 8161 71-5809,Email: Poststelle@LfL.bayern.de,Related OrganizationBayerische Landesanstalt für Landwirtschaft ()Academic or research instituteVöttinger Strasse 38Freising, Bayern
85354, GermanyPhone: + 49 8161 71-5800,Fax: + 49 8161 71-5809,Email: Poststelle@LfL.bayern.de,
Solanum tuberosum was transformed with a construct containing a 581 bp long fragment of exon 2 of the potato granule bound starch synthase (GBSS) gene in antisense orientation. The transcription of this fragment is controlled by its native, tuber-specific promoter P-gbss and its contiguous non-translated leader sequence – consisting of exon 1 and intron 1 of the GBSS gene. The transcription is terminated by the CaMV 35S terminator from Cauliflower mosaic virus.
The markergen-free amylopectin potato pMFIp9-1, line #1332 exhibits an altered starch quality compared to wild-type potato. Nearly pure amylopectin-starch is synthesized.
By introduction of an antisense-sequence the endogenous GBSS gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.
This transgenic organism does not contain any marker genes for selection purposes within the inserted T-DNA.
EN
The markergen-free amylopectin potato pMFIp9-1, line #1332 exhibits an altered starch quality compared to wild-type potato. Nearly pure amylopectin-starch is synthesized.
By introduction of an antisense-sequence the endogenous GBSS gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.
This transgenic organism does not contain any marker genes for selection purposes within the inserted T-DNA.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Solanum tuberosum L. of cultivar Walli transformed
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BPS-25271-9 - Amflora™Potato| Reduced amylose in starch content, Resistance to antibiotics (Kanamycin)
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Potato with modified starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Imidazolinone, Sulfonylurea)
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Potato modified for altered starch composition| AVEBE | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Reduced amylose in starch content
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BPS-A1Ø2Ø-5 - Amadea potato| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Imidazolinone, Sulfonylurea), Selectable marker genes and reporter genes
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Potato with altered starch content| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Sulfonylurea)
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AVE-436G7-1 - Modena potato with altered starch| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
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Potato with altered starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
pMFIp9-1
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- Agrobacterium-mediated DNA transfer
0.000 kb
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581.000 kb
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0.000 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
The GBSS gene is silenced post-transcriptionally.
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- Food
- Feed
- Research
PCR-based methods, to be developed on information on the transformation vector.
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EN
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