Potato with altered starch quality | BCH-LMO-SCBD-103636 | Living Modified Organism | Biosafety Clearing-House

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BCH-LMO-SCBD-103636-2   |   PDF   |   Print   |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 19 Jun 2012 last updated: 28 Jun 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato with altered starch quality
EN
pMFIp9-1, line #1332
No
Solanum tuberosum was transformed with a construct containing a 581 bp long fragment of exon 2 of the potato granule bound starch synthase (GBSS) gene in antisense orientation. The transcription of this fragment is controlled by its native, tuber-specific promoter P-gbss and its contiguous non-translated leader sequence – consisting of exon 1 and intron 1 of the GBSS gene. The transcription is terminated by the CaMV 35S terminator from Cauliflower mosaic virus.

The markergen-free amylopectin potato pMFIp9-1, line #1332 exhibits an altered starch quality compared to wild-type potato. Nearly pure amylopectin-starch is synthesized.

By introduction of an antisense-sequence the endogenous GBSS gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.

This transgenic organism does not contain any marker genes for selection purposes within the inserted T-DNA.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum L. of cultivar Walli transformed
EN
  • BPS-25271-9 - Amflora™Potato
    | Reduced amylose in starch content, Resistance to antibiotics (Kanamycin)
  • Potato with modified starch composition
    | BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Imidazolinone, Sulfonylurea)
  • Potato modified for altered starch composition
    | AVEBE | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Reduced amylose in starch content
  • BPS-A1Ø2Ø-5 - Amadea potato
    | BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Imidazolinone, Sulfonylurea), Selectable marker genes and reporter genes
  • Potato with altered starch content
    | BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Sulfonylurea)
  • AVE-436G7-1 - Modena potato with altered starch
    | BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
  • Potato with altered starch composition
    | BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
Characteristics of the modification process
pMFIp9-1
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | altered carbohydrate composition: increased amylopectin content
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
EN
LMO characteristics
  • BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | altered carbohydrate composition: increased amylopectin content
The GBSS gene is silenced post-transcriptionally.
EN
  • Food
  • Feed
  • Research
Detection method(s)
PCR-based methods, to be developed on information on the transformation vector.
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1