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Modified Organism
Potato with modified starch composition
Record information and status
Record ID
103651
Status
Published
Date of creation
2012-06-28 11:05 UTC (german_bch@bvl.bund.de)
Date of last update
2012-06-28 11:05 UTC (german_bch@bvl.bund.de)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with modified starch composition
Transformation event
Potato (Solanum tuberosum) pHAS3
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
This genetically modified potato exhibits an altered starch metabolism and tolerance towards herbicides containing the active ingredient imazamox.

The inserted DNA segments from plasmid pHAS3 contain fragments of the coding region of the be1 and be2 gene as a double inverted repeat which is regulated by the gbss promoter and the nos terminator from Agrobacterium tumefaciens. This mediates the expression of a RNAi construct that promotes silencing of the endogeneous be1 and be2-gene. A lack of be1 and be2 activity leads to an increased portion of amylopectin within the potato starch.

As a selection marker system the ahas gene from Arabidopsis thaliana under the control of the nos promoter and nos terminator mediates tolerance towards herbicides containing the active ingredient imazamox.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato
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Selectable marker genes and reporter genes
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Characteristics of the transformation process
Vector
pHAS3 (derivative of pPZP200)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Granule bound starch synthase gene promoter - Solanum tuberosum - Potato
Branching Enzyme 1 - Solanum tuberosum - Potato
altered carbohydrate composition: increased amylopectin content
Branching Enzyme 2 - Solanum tuberosum - Potato
altered carbohydrate composition: increased amylopectin content
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium
Acetohydroxy acid synthase - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis
Resistance to herbicides - Imidazolinone, Sulfonylurea
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Branching Enzyme 1 - Solanum tuberosum - Potato
altered carbohydrate composition: increased amylopectin content
Branching Enzyme 2 - Solanum tuberosum - Potato
altered carbohydrate composition: increased amylopectin content
How the expression of the gene(s) was affected
A double-stranded RNA is formed in the genetically modified plants; RNA interference causes inactivation of the endogenous transcripts of the respective genes. As a result, formation of the corresponding enzymes is blocked and a starch with reduced amylopectin content is synthesised in the tubers.
Common use(s)
  • Food
  • Feed
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information on the transformation vector.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record

   
   
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