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Modified Organism
Cisgenic apple trees with scab resistance
Record information and status
Record ID
103938
Status
Published
Date of creation
2012-09-25 12:19 UTC (marco.gielkens@rivm.nl)
Date of last update
2015-07-28 14:27 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-07-28 14:27 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cisgenic apple trees with scab resistance
Transformation event
HcrVf2
Developer(s)
Plant Research International
Dept. Genetics and Breeding
Plant Research International (PRI)
PO Box 59
Wageningen
Netherlands, 6700 AB
Phone:+31 (0)317 48 36 80
Email:info@wur.nl
Url:http://www.wur.nl/uk/
Description
Resistance to the fungus Venturia inaequalis, causative agent of the disease called apple scab, is the trait that was introduced. No further marker or other genes are present.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Malus domestica - Apple, MALDO
Related LMOs
Non flowering apple tree, modified for resistance to fungi
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Fungi Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pMF1
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Homologues of Cladosporium fulvum Resistance genes of the Vf region - Malus domestica - Apple, MALDO
Resistance to diseases and pests - Fungi
Notes regarding the genetic elements introduced or modified in this LMO
The recombinase located on the pMF1 backbone can be activated by adding dexamethason resulting in the excision of the vector backbone. As a result of this excision only the intended trait will be present in the LMO.

Three distinct constructs were generated:
SPVf2: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
CH12: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
RbcVf2: RB --> Apple Rubisco promoter --> HcrVf2 --> Apple Rubisco Terminator --> LB 
LMO characteristics
Modified traits
Common use(s)
  • Research

Records referencing this document (1)
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1record(s) found
Risk Assessment1 record