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Modified Organism
Flax modified for insertional mutagenesis
Record information and status
Record ID
104315
Status
Published
Date of creation
2012-10-24 15:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-10-24 15:47 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Flax modified for insertional mutagenesis
Transformation event
Unknown
Developer(s)
Agritec Plant Research
Agritec Plant Research
Zemedelska 2520/16
Sumperk
Czech Republic, 787 01
Phone:+420 583 382-111
Fax:+420 583 382-999
Email:info@agritec.cz
Url:Agritec Ltd.
Description
Flax and linseed plants were genetically modified to induce mutations of existing plant genes using a vector for insertion mutagenesis. The vector contained a 35S tetramer enhancer fragment which, upon insertion, can cause an activation of the genes adjacent to the T-DNA insertion place, i.e. a dominant mutation. On the other hand the T-DNA integration into an active gene area may cause the inactivation of this gene. This change is then expressed as a recessive mutation. Theoretically these random mutations may result in changes in the plant's metabolism and in specific cases of it's morphological characteristics or the biological properties which may be useful for crop improvement.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Linum usitatissimum - Flax, Flax, Linseed, LINUS
Characteristics of the transformation process
Vector
Binary vector GV3101 (pPM90RK)(pPCVRN4)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Gene 4 transcription terminator
0.00 Kb
 
 
CaMV 35S promoter plus four repeats of activating sequence
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The DNA segment inserted by the binary vector GV3101 (pPM90RK)(pPCVRN4) - does not contain any gene with direct effect on the recipient properties, other than a selectable marker, hpt, that confers resistance to hygromycin for the identification of transformed genotypes.
LMO characteristics
Modified traits
  • Changes in physiology and/or production
Common use(s)
  • Research
Additional Information
Other relevant website address or attached documents

Records referencing this document (1)
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