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Modified Organism
Flax modified for altered fatty acid composition
Record information and status
Record ID
104324
Status
Published
Date of creation
2012-10-24 13:07 UTC (hana_jirakova@env.cz)
Date of publication
2012-10-25 16:39 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Flax modified for altered fatty acid composition
Transformation event
Transformation of flax aimed at fatty acid desaturase 2 (FAD2) gene inactivation by RNA interference
Developer(s)
Agritec Plant Research
Agritec Plant Research
Zemedelska 2520/16
Sumperk
Czech Republic, 787 01
Phone:+420 583 382-111
Fax:+420 583 382-999
Email:info@agritec.cz
Url:Agritec Ltd.
Description
Flax plants were transformed with a vector construct bearing expression cassette with two FAD2 gene (coding for type 2 fatty acids desaturase) segments modified in a way to employ RNA interference system for suppression of desaturase function, and due to it to increase the content of oleic acid in linseed oil.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Linum usitatissimum - Flax, Flax, Linseed, LINUS
Characteristics of the transformation process
Vector
pPCV812i/35S/FAD2i/35S/GUS (FAD2-i GUS)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Hygromycin B phosphotransferase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Hygromycin
Selectable marker genes and reporter genes
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Fatty Acid Desaturase 2 - Linum usitatissimum - Flax, Flax, Linseed, LINUS
Changes in quality and/or metabolite content - Lipid and fatty acids
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Beta-lactamase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Ampicillin
Notes regarding the genetic elements introduced or modified in this LMO
The inserted plasmid construct contains the FAD2-i expression cassette (the CaMV 35S promoter, DNA segment for desaturase, spacer, inverted DNA segment), CaMV 35S promoter from pAN4 plasmid, hpt gene from E. Coli, uidA gene for beta-glucuronidase (GUS) together with E.coli intron (K12) and AmpR gene for plasmid selection in bacteria.

Along with modified FAD2 gene the insert contains hpt selectable gene to enable selection of transgenic plants, uidA (GUS) reporter gene and AmpR gene to support plasmid construct selection in bacteria.
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Other relevant website address or attached documents

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