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Modified Organism
Barley modified to produce phytase
Record information and status
Record ID
104335
Status
Published
Date of creation
2012-10-31 13:07 UTC (hana_jirakova@env.cz)
Date of publication
2012-10-31 20:52 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Barley modified to produce phytase
Transformation event
SCLW-GP-PHYA
Developer(s)
Dr. Ludmila Ohnoutková
senior scientist
Institute of Experimental Botany AS CR (IEB)
Prague
Czech Republic
Phone:+420 225 106 455
Fax:+420 225 106 456
Email:ueb@ueb.cas.cz
Url:Institute of Experimental Botany AS CR
Description
SCLW-GP-PHYA barley has been genetically modified by insertion of the fungal phytase gene from Aspergillus niger. Cereals (including barley), oil crops and legumes contain phytic acid, myo-inositolhexakisphosphate that is an antinutritional compound. Ions of iron and cations (manganese, magnesium, zinc and calcium) which are bound to phytic acid are difficult to digest. These complexes negatively affect the usability of phosphorus and bound cations in human and monogastric animal foodstuffs. The enzyme 3-phytase is effectively releasing phosphorus and cations which are bound to phytic acid in barley seeds. The higher-phytase lines show 3.5 fold increase content of pytase activity in barley grain.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Point of collection or acquisition of the recipient organism
Variety: Golden Promise
Characteristics of the transformation process
Vector
Cotransformation of pAMFIT and pAL70 vectors
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Alpha-amylase gene promoter
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phyA gene
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Zygotic immature embryos of spring barley were co-transformed by particle bombardment with combinations of two vectors:

The pAMFIT vector contains the gene phyA from Aspergillus niger.

The pAL70 vector contains the herbicide selection gene (bar) from Streptomyces hygroscopicus, conferring resistance against the plant herbicide Basta, and the common luciferase reporter gene (luc) from Photinus pyralis.

Transgenic plants were regenerated from embryogenic callus selected on Bialaphos containing medium, and analyzed for presence phyA, bar, luc genes by PCR and RT-PCR.

Notes on Genetic Elements:
α-amylase promoter: promoter region from Hordeum vulgare cv. Himalaya gene
bar gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
luc gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

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