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Modified Organism
EMB-PVØ51-1 - Common bean modified for resistance to Bean Golden Mosaic Virus
Record information and status
Record ID
104605
Status
Published
Date of creation
2013-01-11 11:56 UTC (gutemberg.sousa@mctic.gov.br)
Date of last update
2015-01-26 18:57 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 18:57 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Common bean modified for resistance to Bean Golden Mosaic Virus
Transformation event
Embrapa 5.1
Unique identifier
EMB-PVØ51-1
Developer(s)
Mr Francisco José Lima Aragão
Cientist
National Research Center of Biotechnology Research
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Parque Estação Biológica - Final da W5 Norte - Caixa Postal 02372
Brasília, Distrito Federal
Brazil, 70770-900
Phone:55 + 61 3448-4710,55 + 61 3448-4706
Fax:55+613340-3658
Email:aragao@cenargen.embrapa.br
Url:http://lattes.cnpq.br/6121745125946782
Mr Josias Correa Farias
Cientist
National Center for Research of Rice and Beans
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Rodovia Goiânia - Nova Veneza, Km 12 - Zona Rural. Caixa Postal 179
Santo Antonio de Goiás, Goiás
Brazil, 75375-000
Phone:55 (62) 3532-2110
Fax:55 (62) 3533-2100
Email:josias@cnpaf.embrapa.br
Url:Lattes Plataform
Description
The common bean (Phaseolus vulgaris L.) was genetically modified to exhibit resistance to the Bean Golden Mosaic Virus (BGMV).

RNA interference (RNAi) technology was used to impart resistance to BGMV by generating a construct containing a fragment of the AC1 gene of BGMV, positioned in sense/ antisense orientation.

This results in the transcript forming a double stranded RNA hairpin that is recognized by cellular machinery and thus initiates the degradation process of the dsRNA fragments. This ultimately results in the silencing of the AC1 gene which is essential for viral genome replication and consequently imparts resistance against the BGMV virus in the plant.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Phaseolus vulgaris - String bean, French bean, Kidney bean, Common Bean , PHAVU
Characteristics of the transformation process
Vector
pBGMVRNAiAHAS derived from vector pBLUESCRIPT II SK (-)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Acetohydroxy acid synthase gene
0.00 Kb
 
 
Acetohydroxy acid synthase gene promoter
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
AC1 Gene
0.41 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.00 Kb
 
 
AC1 Gene
0.41 Kb
 
 
Octopine Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The pBGMVRNAiAHAS vector also contained the beta-lactamase gene of Escherichia coli, this was however shown to be non functional in the Embrapa 5.1 line.
Three copies of the AHAS gene integrated into the host genome however the expression of this gene occurs at a level that is considered too low to impart significant herbicide tolerance in the Embrapa 5.1 line.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record

   
   
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